Investigating the effects of psychosocial stress on laryngeal microbiology and epithelial barrier integrity
研究社会心理压力对喉部微生物学和上皮屏障完整性的影响
基本信息
- 批准号:10824680
- 负责人:
- 金额:$ 7.08万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-01 至 2025-08-31
- 项目状态:未结题
- 来源:
- 关键词:16S ribosomal RNA sequencingAcousticsAerobicBacteriaBacterial TranslocationBiologicalBiologyBrainChronicCollectionDataDevelopmentDown-RegulationEpithelial Cell JunctionEpitheliumExhibitsExposure toFellowshipFluorescent in Situ HybridizationFunctional disorderFutureGene ExpressionGenesImmunofluorescence ImmunologicImmunohistochemistryInfectionKnowledgeLaryngeal EpitheliumLarynxLeadLinkMediatingMembraneMethodologyMicrobeMicrobiologyMucinsMucous MembraneMucous body substanceMusNational Research Service AwardsOrganOutcomePathologyPatientsPeriodicalsPermeabilityPersonality TraitsPolymerase Chain ReactionPredispositionProbioticsProliferatingProtocols documentationPsychological StressPsychosocial InfluencesPsychosocial StressReportingResearchRoleSamplingSocial BehaviorStainsStomachStressStressful EventTherapeutic InterventionThickTimeTissuesTreatment/Psychosocial EffectsVoiceVoice Disordersbiological adaptation to stressdysbiosisexperimental studygut microbiotainjury recoverymechanical stimulusmicrobialmicrobial communitymicrobial compositionmicrobial hostmicrobiomemicrobiotamouse modelmucosal microbiotapathogenic bacteriaprobiotic therapyresponserestraint stresssexskillstargeted treatmentvocal cord
项目摘要
ABSTRACT
Biological mechanisms underlying psychosocial stress-induced changes to the larynx remain understudied.
Psychological stress symptomology is concurrently reported in 25% of patients with voice problems. From a
biological perspective, psychosocial stress can have deleterious consequences on both microbial communities
and epithelial barrier integrity, as reported, in other mucosal organs such as the gut and stomach. First, the
laryngeal microbiome has a distinct microbial composition when compared to the gut. How this distinct
composition is influenced by psychosocial stress remains unknown. Independently, stress-induced changes to
epithelial permeability (such as reduced epithelial proliferation and reduced mucus thickness) may potentially
leave the larynx susceptible to further infection. This proposal broadly questions whether psychosocial stress
can lead to microbial dysbiosis and compromised epithelial barrier integrity. In our proposed experiments, mice
will be assigned to unstressed or stressed groups. Psychosocial stress will be induced via a validated chronic
restraint stress protocol. Specific Aim 1 will determine laryngeal microbial adaptations following chronic,
psychosocial stress exposure. We hypothesize that psychosocial stress will result in reduced microbial
diversity and abundance, altered composition and differential abundance of specific bacterial taxa, and distinct
predicted functional profiles in the laryngeal microbiota. To obtain laryngeal microbiota outcomes, 16s rRNA
sequencing will be used completed on bacteria extracted from murine laryngeal tissue. Specific Aim 2 will
delineate changes in vocal fold epithelial integrity post-chronic psychosocial stress. We hypothesize
that psychosocial stress will result in increased bacterial translocation, decreased mucus thickness, reduced
epithelial proliferation, reduced epithelial cell junction integrity, and altered gene expression of mucins in the
vocal folds. Immunohistochemistry/ Immunofluorescence and qPCR will be used to delineate consequences of
psychosocial stress on the laryngeal epithelium. Taken together, we hypothesize that psychosocial stress will
concomitantly lead to microbial dysbiosis and alter epithelial barrier integrity in the larynx. Data from these two
aims will lay the groundwork for identifying and manipulating specific bacterial phyla to delineate host-microbial
epithelial interactions of psychosocial stress in the larynx.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
ANUMITHA VENKATRAMAN其他文献
ANUMITHA VENKATRAMAN的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('ANUMITHA VENKATRAMAN', 18)}}的其他基金
Investigating the role of systemic dehydration in vocal fold wound healing
研究全身脱水在声带伤口愈合中的作用
- 批准号:
10188367 - 财政年份:2020
- 资助金额:
$ 7.08万 - 项目类别:
相似海外基金
Nonlinear Acoustics for the conditioning monitoring of Aerospace structures (NACMAS)
用于航空航天结构调节监测的非线性声学 (NACMAS)
- 批准号:
10078324 - 财政年份:2023
- 资助金额:
$ 7.08万 - 项目类别:
BEIS-Funded Programmes
ORCC: Marine predator and prey response to climate change: Synthesis of Acoustics, Physiology, Prey, and Habitat In a Rapidly changing Environment (SAPPHIRE)
ORCC:海洋捕食者和猎物对气候变化的反应:快速变化环境中声学、生理学、猎物和栖息地的综合(蓝宝石)
- 批准号:
2308300 - 财政年份:2023
- 资助金额:
$ 7.08万 - 项目类别:
Continuing Grant
University of Salford (The) and KP Acoustics Group Limited KTP 22_23 R1
索尔福德大学 (The) 和 KP Acoustics Group Limited KTP 22_23 R1
- 批准号:
10033989 - 财政年份:2023
- 资助金额:
$ 7.08万 - 项目类别:
Knowledge Transfer Partnership
User-controllable and Physics-informed Neural Acoustics Fields for Multichannel Audio Rendering and Analysis in Mixed Reality Application
用于混合现实应用中多通道音频渲染和分析的用户可控且基于物理的神经声学场
- 批准号:
23K16913 - 财政年份:2023
- 资助金额:
$ 7.08万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Combined radiation acoustics and ultrasound imaging for real-time guidance in radiotherapy
结合辐射声学和超声成像,用于放射治疗的实时指导
- 批准号:
10582051 - 财政年份:2023
- 资助金额:
$ 7.08万 - 项目类别:
Comprehensive assessment of speech physiology and acoustics in Parkinson's disease progression
帕金森病进展中言语生理学和声学的综合评估
- 批准号:
10602958 - 财政年份:2023
- 资助金额:
$ 7.08万 - 项目类别:
The acoustics of climate change - long-term observations in the arctic oceans
气候变化的声学——北冰洋的长期观测
- 批准号:
2889921 - 财政年份:2023
- 资助金额:
$ 7.08万 - 项目类别:
Studentship
Collaborative Research: Estimating Articulatory Constriction Place and Timing from Speech Acoustics
合作研究:从语音声学估计发音收缩位置和时间
- 批准号:
2343847 - 财政年份:2023
- 资助金额:
$ 7.08万 - 项目类别:
Standard Grant
Flow Physics and Vortex-Induced Acoustics in Bio-Inspired Collective Locomotion
仿生集体运动中的流动物理学和涡激声学
- 批准号:
DGECR-2022-00019 - 财政年份:2022
- 资助金额:
$ 7.08万 - 项目类别:
Discovery Launch Supplement
Collaborative Research: Estimating Articulatory Constriction Place and Timing from Speech Acoustics
合作研究:从语音声学估计发音收缩位置和时间
- 批准号:
2141275 - 财政年份:2022
- 资助金额:
$ 7.08万 - 项目类别:
Standard Grant