Statistical and high-throughput models of enhancer function and evolution

增强子功能和进化的统计和高通量模型

• 批准号: 10846199
• 负责人: SCOTT V. EDWARDS
• 金额: $ 2.63万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-07 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10846199
• 关键词: African American; Award; Biological Models; Biology; Comparative Biology; Computer software; Data Set; Diving; Ducks; Elements; Enhancers; Ensure; Evolution; Forelimb; Funding; Genetic; Genome; Goals; Home; Institution; Laboratories; Link; Mentors; Modeling; Molecular Evolution; Morphology; Parents; Phenotype; Phylogenetic Analysis; Publications; Research; Scanning; Sternum; Technical Expertise; Texas; Trees; Universities; Variant; Vertebrates; career development; comparative genomics; cranium; digital; genome analysis; parent grant; phenome; programs; skeletal; skills; trait; whole genome

Project Summary This diversity supplement will help equip Dr. Janet Buckner, an African American evolutionary ornithologist at the University of Texas Arlington, with cutting-edge skills in comparative genomics and genome biology. Dr. Buckner currently leads a vibrant research program in comparative biology, with an emphasis on phylogenetics, molecular evolution and documenting and understanding convergent evolution, primarily in vertebrates. However, she has yet to embrace whole-genome analyses that will allow her to associate genome variation and phenotypic variation using phylogenetic trees. In this supplement, we accomplish these goals by expanding current data sets in our respective labs to allow genome-phenome analysis using convergent evolution in diving ducks (Anatidae) as a model system. Deep diving in ducks is well known to have evolved multiple times and provides an excellent setting to link genome variation and phenotypic variation. Both Dr. Buckner and the PI have been amassing data sets on digitally scanned morphological variation in multiple skeletal elements in ducks, including the skull, hind- and forelimb elements, and the sternum. Additionally, the PI has been assembling whole- genome alignments of ducks as part of Aim 2 of the parent award, because ducks have multiple high-quality genomes available online and because the group displays substantial morphological variation whose genetic basis is unknown. We will bring together whole-genome alignments and both discrete and continuous morphological trait variation in ducks using our core software, PhyloAcc, which has been extended in multiple ways as part of the parent grant. Additionally, Dr. Buckner will receive mentoring and career development strategies to increase her competitiveness in applications for federal funding and to strengthen her publications to ensure achieving tenure at her home institution and increasing her scientific impact. Together these new technical skills and mentoring will allow Dr. Buckner and her laboratory to broaden her approaches in comparative biology and significantly enhance her competitiveness in the funding arena.

项目摘要 这种多样性补充将有助于装备珍妮特巴克纳博士，一个非洲裔美国人， 德克萨斯大学阿灵顿分校的进化鸟类学家，在 比较基因组学和基因组生物学。巴克纳博士目前领导着一个充满活力的 比较生物学研究计划，重点是遗传学，分子生物学 进化和记录和理解趋同进化，主要是在 脊椎动物然而，她还没有接受全基因组分析， 她使用系统发育树将基因组变异和表型变异联系起来。在 在本补充中，我们通过扩展我们的 各自的实验室允许使用潜水中的趋同进化进行基因组-表型组分析 鸭（鸭科）作为一个模式系统。众所周知，鸭子的深潜进化 并提供了一个很好的设置链接基因组变异和表型 变化量巴克纳博士和私家侦探都在收集数字扫描的数据集 鸭的多种骨骼元素的形态变异，包括头骨，后， 前肢和胸骨此外，PI一直在组装整个- 作为父母奖目标2的一部分，鸭子的基因组比对，因为鸭子有 多个高质量的基因组可以在网上获得， 遗传基础未知的大量形态学变异。我们将带来 结合全基因组比对以及离散和连续形态性状 使用我们的核心软件PhyloAcc进行鸭的变异，该软件已扩展到多个 作为家长补助金的一部分。此外，巴克纳博士将接受指导， 职业发展战略，以提高她的竞争力，在申请联邦 资助并加强她的出版物，以确保在她的家中实现终身教职 并提高其科学影响力。结合这些新的技术技能， 指导将使巴克纳博士和她的实验室扩大她的方法， 比较生物学，并显着提高她在资金竞技场的竞争力。