Statistical and high-throughput models of enhancer function and evolution

增强子功能和进化的统计和高通量模型

• 批准号: 10846199
• 负责人: SCOTT V. EDWARDS
• 金额: $ 2.63万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-07 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10846199
• 关键词: African American; Award; Biological Models; Biology; Comparative Biology; Computer software; Data Set; Diving; Ducks; Elements; Enhancers; Ensure; Evolution; Forelimb; Funding; Genetic; Genome; Goals; Home; Institution; Laboratories; Link; Mentors; Modeling; Molecular Evolution; Morphology; Parents; Phenotype; Phylogenetic Analysis; Publications; Research; Scanning; Sternum; Technical Expertise; Texas; Trees; Universities; Variant; Vertebrates; career development; comparative genomics; cranium; digital; genome analysis; parent grant; phenome; programs; skeletal; skills; trait; whole genome

Project Summary This diversity supplement will help equip Dr. Janet Buckner, an African American evolutionary ornithologist at the University of Texas Arlington, with cutting-edge skills in comparative genomics and genome biology. Dr. Buckner currently leads a vibrant research program in comparative biology, with an emphasis on phylogenetics, molecular evolution and documenting and understanding convergent evolution, primarily in vertebrates. However, she has yet to embrace whole-genome analyses that will allow her to associate genome variation and phenotypic variation using phylogenetic trees. In this supplement, we accomplish these goals by expanding current data sets in our respective labs to allow genome-phenome analysis using convergent evolution in diving ducks (Anatidae) as a model system. Deep diving in ducks is well known to have evolved multiple times and provides an excellent setting to link genome variation and phenotypic variation. Both Dr. Buckner and the PI have been amassing data sets on digitally scanned morphological variation in multiple skeletal elements in ducks, including the skull, hind- and forelimb elements, and the sternum. Additionally, the PI has been assembling whole- genome alignments of ducks as part of Aim 2 of the parent award, because ducks have multiple high-quality genomes available online and because the group displays substantial morphological variation whose genetic basis is unknown. We will bring together whole-genome alignments and both discrete and continuous morphological trait variation in ducks using our core software, PhyloAcc, which has been extended in multiple ways as part of the parent grant. Additionally, Dr. Buckner will receive mentoring and career development strategies to increase her competitiveness in applications for federal funding and to strengthen her publications to ensure achieving tenure at her home institution and increasing her scientific impact. Together these new technical skills and mentoring will allow Dr. Buckner and her laboratory to broaden her approaches in comparative biology and significantly enhance her competitiveness in the funding arena.

项目摘要 这一多样性补充将有助于装备非裔美国人珍妮特·巴克纳博士 德克萨斯大学阿灵顿分校的进化鸟类学家，在 比较基因组学和基因组生物学。巴克纳博士目前领导着一项充满活力的 比较生物学研究计划，重点是系统发育学、分子生物学 进化以及记录和理解融合进化，主要是在 脊椎动物。然而，她还没有接受全基因组分析，这将使 用系统发育树将基因组变异和表型变异联系起来。在……里面 作为补充，我们通过扩展我们现有的数据集来实现这些目标 各自的实验室将允许在潜水中使用收敛进化进行基因组-表现组分析 鸭科作为模式系统。众所周知，鸭子的深度潜水是由 并提供了将基因组变异和表型联系起来的极佳环境 变种。巴克纳博士和私家侦探都在收集数字扫描的数据集 鸭多种骨骼元素的形态变异，包括头骨、后肢和 和前肢元素，还有胸骨。另外，私家侦探一直在组装- 作为亲本奖目标2的一部分，鸭子的基因组比对，因为鸭子有 多个高质量的基因组可以在线获得，因为该组织展示了 巨大的形态变异，其遗传基础未知。我们会带来 全基因组比对以及离散和连续的形态特征 使用我们的核心软件PhyloAcc对鸭子进行了变异，该软件已扩展到多个 作为父母资助的一部分。此外，巴克纳博士将接受指导和 职业发展战略，以增加她在联邦政府申请中的竞争力 资助并加强她的出版物，以确保在家中实现终身教职 并增加了她的科学影响力。将这些新的技术技能和 指导将允许巴克纳博士和她的实验室拓宽她在 比较生物学，并显著提高了她在资金领域的竞争力。