Administrative Diversity Supplement for Ta-Tyonna Buck.

项目摘要：在侵入性脑外科手术中，硬脑膜被破坏， 严重的术后并发症，如果不及时治疗可能是致命的。市售硬脑膜移植物 表现欠佳，迫切需要硬脑膜移植替代物， 结构完整性和重新整合，炎症和术后 并发症父母补助金将追求具体目标，以评估以下方面的有效性： 硬脑膜移植物替代硬脑膜移植物置换。在这里，我们提出了补充目标， 研究星形胶质细胞在硬脑膜移植物再融合后愈合中的作用。实验 补充项目的目标将被纳入父母补助金的方法， 提高我们对硬脑膜移植物替代物的必要质量的理解， 重新融入社会 总的目标是让Ta-Tyonna Buck女士研究星形胶质细胞的作用， 它们对神经修复的贡献在补充目标1中，Ta-Tyonna将利用 评估星形胶质细胞在硬脑膜移植物上的分子机制的体外模型 通过3D共培养系统进行替代。在补充目标2中，她将评估以下方面的作用： 星形胶质细胞在体内模型中测量硬脑膜损伤后星形胶质细胞的影响和反应性 移植重组 Ta-Tyonna将获得生物医学成像和显微镜方面的广泛技能，所有这些都是 需要在拟议目标方面取得进展。塔-泰安娜每周都会和威廉姆斯博士见面， 威廉姆斯实验室的指导，研究更新，反馈和审查更新 文学此外，Ta-Tyonna将每月与父母补助金调查团队会面， 指导和更新所有研究。她将参加研讨会的研究和写作 成功用友，参加全国会议，介绍她的工作，并指导本科生 在威廉姆斯实验室进行研究此外，她将参加专业发展 在会议和研究研讨会上开展活动，以利用其许多网络联系 和学习机会。研究和学习活动的结合， 战略概述，以支持塔-Tyonna的整个博士学位的进展。当她追求 从事研究工作

Project Summary: Cranial dura mater is disrupted during invasive brain surgeries, resulting in major post-surgical complications that may be fatal if left untreated. Commercial dura grafts perform sub-optimally, creating a critical need for a dura graft substitute that maintains structural integrity and reintegrates with minimal inflammation and post-surgical complications. The parent grant will pursue specific aims to evaluate the effectiveness of dura graft substitutes for dura graft replacement. Here we propose supplemental aims to investigate the role of astrocytes in healing following dura graft reintegration. The experiments and goals of the supplemental project will be incorporated into the parent grant’s methods to advance our understanding of the necessary qualities of dura graft substitutes for effective reintegration. The overall goal is for Ms. Ta-Tyonna Buck to investigate the role of astrocytes and the contributions they have to neural healing. In Supplemental Aim 1, Ta-Tyonna will utilize an in vitro model to assess the molecular mechanisms of astrocytes on the dura graft substitutes via a 3D co-culture system. In Supplemental Aim 2, she will assess the role of astrocytes in an in vivo model to measure the impact and reactivity of astrocytes following dura graft reintegration. Ta-Tyonna will gain an extensive skillset in biomedical imaging and microscopy, all of which are needed to progress in the proposed goals. Ta-Tyonna will meet weekly with Dr. Williams and the Williams lab for mentoring, research updates, feedback, and reviewing updated literature. Additionally, Ta-Tyonna will meet monthly with the parent grant investigator team for mentoring and updates on all studies. She will participate in workshops for research and writing success at UF, attend national conferences to present her work, and mentor undergraduates conducting research in the Williams Lab. Furthermore, she will attend professional development activities at conferences and research symposia to take advantage of their many networking and learning opportunities. The combined research and learning activities have been strategically outlined to support Ta-Tyonna’s progress throughout her Ph.D. and as she pursues a career in research.