The Broad-LMM-Color Genome Center for All of Us

适合所有人的宽 LMM 颜色基因组中心

• 批准号: 10884763
• 负责人: Stacey Gabriel
• 金额: $ 3430万
• 依托单位: BROAD INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-25 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10884763
• 关键词: Aliquot; All of Us Genome Center; All of Us Research Program; Automation; Biological Assay; Color; Data; Ensure; Environment; Genotype; Laboratories; Participant; Process; Quality Control; Sampling; Testing; Validation; biobank; cost

The All of Us Research Program ( AoURP) requires a robust, accurate, and thoroughly CLIA validated sample receipt process, with the capacity to process ~17,000 samples per month at high-quality. To date, the Samples Lab has received, accessioned, and stored over 2 million samples (Figure 2) and has been tested at scale with large national biobanks. To ensure high-quality at high-scale, our processes leverage highly optimized automation with full LIMS tracking to ensure chain of custody and apply various quality control (QC) assays on all incoming samples. We use the same process to receive samples for both genotyping and sequencing. This streamlined approach—that is, performing genotyping and WGS in a single CLIA lab environment—reduces the cost of cross-laboratory sample-identity checking, enables seamless cross-validation of sequencing data with genotype data, and conserves material by allowing genotyping and sequencing to be performed on a single aliquot of a participant’s sample.

我们所有的研究计划（AoURP）需要一个强大的，准确的，彻底的CLIA 经过验证的样品接收流程，每月可高质量处理约17，000个样品。 到目前为止，Samples Lab已经接收、访问和存储了超过200万个样本（图2）， 在大型国家生物库中进行了大规模测试。为了确保大规模的高质量，我们的流程 利用高度优化的自动化和完整的LIMS跟踪，以确保监管链，并应用各种 所有来料样品的质量控制（QC）分析。我们使用相同的流程接收两种产品的样品 基因分型和测序。这种简化的方法-即在一个单一的基因分型和WGS中进行基因分型， CLIA实验室环境-降低跨实验室样品身份检查的成本， 测序数据与基因型数据的交叉验证，并通过允许基因分型和 在参与者的样品的单个等分试样上进行测序。