Mechanisms of cohesin regulation in vertebrates

脊椎动物粘连蛋白调节机制

• 批准号: 10855552
• 负责人: Susannah Rankin
• 金额: $ 6.13万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-03 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10855552
• 关键词: Basic Science; Biological Process; Cell division; Cells; Chromatography; Chromosome Segregation; Chromosomes; DNA Repair; DNA biosynthesis; Development; Ensure; Equipment; Foundations; Funding; Genetic Transcription; Mediating; Mission; Molecular; Molecular Genetics; National Institute of General Medical Sciences; Nuclear Structure; Outcome; Parents; Performance; Play; Prevention; Proteins; Regulation; Request for Applications; Role; Sister Chromatid; System; Testing; Vertebrates; Work; cohesin; cohesion; disease diagnosis; genetic approach; parent grant; protein complex; protein purification

Abstract In vertebrate cells the cohesin protein complex plays critical roles in nuclear structure and function. It tethers together the identical products of DNA replication, called sister chromatids, until cell division and it also mediates intra-chromosomal bridging interactions, forming chromosome loops and domains. While cohesion between sister chromatids is critical for accurate chromosome segregation and certain kinds of DNA repair, the compaction of chromosomes into loops and domains is essential for proper transcription and normal development. How these different kinds of cohesion differ at the molecular level, the mechanisms that ensure each outcome, and the amount of overlap between them are not well understood. Using molecular genetic approaches, we are testing the impacts of specific interactions, in multiple experimental systems, on cohesin regulation. In this application we are requesting supplemental funds to purchase chromatography equipment that will enhance our efficiency in purification and characterization of proteins to complete the projects described in the parent proposal.

摘要 在脊椎动物细胞中，粘附蛋白复合物在细胞核结构中起关键作用， 功能它将DNA复制的相同产物连接在一起，称为姐妹染色单体， 直到细胞分裂，它也介导染色体内的桥接相互作用，形成 染色体环和结构域。虽然姐妹染色单体之间的凝聚力对于 精确的染色体分离和某些类型的DNA修复， 染色体的环和结构域是必要的适当转录和正常 发展这些不同种类的凝聚力在分子水平上是如何不同的， 确保每一项成果的机制，以及它们之间的重叠量， 明白利用分子遗传学方法，我们正在测试特定的 相互作用，在多个实验系统中，对cohesin调节。在这个应用程序中，我们 要求补充资金购买色谱设备，这将提高我们的 蛋白质的纯化和表征效率，以完成 父母的建议。