FUNGAL FERRITINS

真菌铁蛋白

• 批准号: 2018072
• 负责人: CARL J CARRANO
• 金额: $ 9.99万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-30 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2018072
• 关键词: Fungi; developmental genetics; ferritin; fungal genetics; gene induction /repression; iron metabolism; microorganism culture; nucleic acid sequence; polymerase chain reaction; protein structure function

As relatively simple eukaryotes, fungi appear to have aspects to their iron metabolism that are related to both other microbial (prokaryotic) systems as well as those of the higher eukaryotes. Thus its study can tell us something of the evolutionary development of iron metabolism as well as providing a simpler model of mammalian iron transport and storage and regulation. In addition, the Zygomycetes group of fungi that we plan to study, contains a number of human fungal pathogens hence a more detailed knowledge of this important part of their metabolism may lead to advances in therapy. In our preliminary work, two distinct ferritin-like iron containing proteins have been identified and isolated from the fungus Absidia spinosa; one from the spores and another from the mycelia. The mycelial protein has been purified and consists of two subunits of Ca. 20 kD molecular weight. Based on the presence of a heme spectrum and the high degree of sequence homology found, it is established that the mycelial protein is a bacterioferritin. This is the first example demonstrating the presence of a bacterioferritin in a eukaryotic organism. The spore protein has not yet been purified to homogeneity but it is distinct by gel electrophoresis from the mycelial ferritin, does not appear to contain heme, and has Mossbauer parameters suggestive of a "mammalian" like ferritin core. We plan to isolate the genes coding for both forms of the fungal ferritins, overexpress them and study some of their biophysical properties related to iron core formation. The observation that the "bacterio" type and "conventional" types of ferritin both exist in fungi but in different developmental forms also opens the way for detailed study of the function and regulation of these two proteins in a way that has not been possible in other classes of organisms.

作为相对简单的真核生物，真菌似乎具有以下方面： 它们的铁代谢与其他微生物有关 （原核）系统以及高等真核生物系统。 因此，它的研究可以告诉我们一些进化论的信息 铁代谢的发展以及提供更简单的模型 哺乳动物铁的运输、储存和调节。在 此外，我们计划研究的接合菌纲真菌， 含有许多人类真菌病原体，因此更详细 了解新陈代谢的这一重要部分可能会导致 治疗方面的进步。在我们的前期工作中，两个不同的 已鉴定出类似铁蛋白的含铁蛋白质 从真菌刺霉 (Absidia spinosa) 中分离出来；一种来自孢子和 另一个来自菌丝体。菌丝蛋白已纯化 并由 Ca 的两个亚基组成。 20 kD 分子量。基于 关于血红素谱的存在和高度序列 发现同源性，确定菌丝蛋白是 细菌铁蛋白。这是第一个示例，展示了 真核生物中存在细菌铁蛋白。孢子 蛋白质尚未纯化至同质，但它是不同的 通过凝胶电泳检测菌丝体铁蛋白，未出现 含有血红素，并且具有穆斯堡尔参数暗示 “哺乳动物”就像铁蛋白核心。我们计划分离编码基因 对于两种形式的真菌铁蛋白，过度表达它们并研究 它们的一些生物物理特性与铁芯有关 形成。观察结果表明“细菌”类型和 “传统”类型的铁蛋白都存在于真菌中，但存在于 不同的发展形式也为详细研究开辟了道路 这两种蛋白质的功能和调节方式 在其他类别的生物体中这是不可能的。