NON-RADIOACTIVE METHOD FOR 25-OH-D & 1,25 (OH)2D IN BLO

描述：(改编自申请人的摘要)主要目标 本研究旨在建立一种高度敏感和特异的检测方法。 维生素D的两种重要代谢物--25-羟基-2 维生素D和1，25二羟基维生素D，在非放射性检测中。 初步研究表明，一种最近合成的生物素- 25-羟基-D3的共轭化合物可用于测定药物浓度 实验室样品中的25-羟基-D3。1，25-(OH)2-D3的生物素结合物 也被合成并用于结合研究。第一阶段 目标将是使用这些最近合成的试剂来开发一种 25-羟基-D和1，25-(OH)2-D3的非放射性测定 在鲜血中。然后，这种方法可以被其他实验室采用。多数 所需试剂的一部分已经合成，研究人员 对之前的大多数担忧都做出了很好的回应。然而，关键是 在提出的实验中，缺乏控制和细节。因此， 虽然这次修改后的申请比前一次更好， 担忧依然存在，该提案只产生了温和的 热情。

DESCRIPTION: (Adapted from applicant's abstract) The primary goal of this research is to develop a highly sensitive and specific assay for the measurement of 2 important metabolites of vitamin D, 25-hydroxy- vitamin D and 1,25 dihydroxy vitamin D, in a non-radioactive assay. Preliminary work has demonstrated that a recently synthesized biotin- conjugate of 25-OH-D3 can be useful for measuring the concentration of 25-OH-D3 in a laboratory sample. A biotin conjugate of 1,25-(OH)2-D3 has also been synthesized and used for binding studies. The Phase I goal will be to use these recently synthesized reagents to develop a non-radioactive assay for quantification of 25-OH-D and 1,25-(OH)2-D3 in blood. This method could then be adopted other laboratories. Most of the reagents required have been synthesized, and the investigator has responded well to most of the previous concerns. However, critical controls and details are lacking in the experiments proposed. Thus, although this revised application is superior than the previous one, concern still exists and the proposal generates only moderate enthusiasm.