NON-RADIOACTIVE METHOD FOR 25-OH-D & 1,25 (OH)2D IN BLO

描述：（改编自申请人的摘要）的主要目标 本研究旨在开发一种高度敏感和特异的检测方法， 维生素D的2种重要代谢产物25-羟基- 维生素D和1，25二羟基维生素D，在非放射性测定。 初步研究表明，最近合成的生物素- 25-OH-D3的缀合物可用于测量25-OH-D3的浓度。 实验室样本中的25-OH-D3。 1，25-（OH）2-D3的生物素缀合物 也被合成并用于结合研究。 i期 我们的目标将是使用这些最近合成的试剂来开发一种 用于定量25-OH-D和1，25-（OH）2-D3的非放射性测定 是血 这种方法可以被其他实验室采用。 最 所需的试剂已经合成，研究人员 对之前的大部分问题都有很好的回应。 然而， 在所提出的实验中缺乏控制和细节。 因此，在本发明中， 尽管该修改的申请比先前的申请优越上级， 关注仍然存在，建议只产生温和的 热情

DESCRIPTION: (Adapted from applicant's abstract) The primary goal of this research is to develop a highly sensitive and specific assay for the measurement of 2 important metabolites of vitamin D, 25-hydroxy- vitamin D and 1,25 dihydroxy vitamin D, in a non-radioactive assay. Preliminary work has demonstrated that a recently synthesized biotin- conjugate of 25-OH-D3 can be useful for measuring the concentration of 25-OH-D3 in a laboratory sample. A biotin conjugate of 1,25-(OH)2-D3 has also been synthesized and used for binding studies. The Phase I goal will be to use these recently synthesized reagents to develop a non-radioactive assay for quantification of 25-OH-D and 1,25-(OH)2-D3 in blood. This method could then be adopted other laboratories. Most of the reagents required have been synthesized, and the investigator has responded well to most of the previous concerns. However, critical controls and details are lacking in the experiments proposed. Thus, although this revised application is superior than the previous one, concern still exists and the proposal generates only moderate enthusiasm.