CARDIAC MICRODIALYSIS, ADENOSINE, AND CARDIAC FUNCTION

描述（改编自申请人的摘要）： 建议的研究是：1）探索心脏的有用性 微透析技术用于心肌间质取样 液体（ISF）和局部冠状动脉血流量（CBF）的测量;以及2） 利用心脏微透析技术研究 腺苷。 在拟议的实验中，心脏微透析，用于 测定ISF中腺苷、肌苷、次黄嘌呤、黄嘌呤、尿酸 酸和乳酸盐将与标准品结合使用。 测量局部心室功能的技术， 代谢进行四个系列的实验。 第1集评估 心脏微透析的有效性、适用性和局限性 法 系列2的实验将利用 心脏微透析技术提供 局部地将药理学试剂施加到微透析周围的组织 探针，用这种方法来研究腺苷的代谢和行动。 在 系列3，心脏微透析将用于研究 局部ISF嘌呤代谢物和乳酸将与变化相关 缺氧时局部CBF和局部心室功能， 刺激和冠状动脉灌注压的分级降低。 在系列4中，心脏微透析将用于研究心肌 缺血，重点是改变内源性腺苷水平的干预措施 在局部缺血期间。

DESCRIPTION (adapted from applicant's abstract): The objectives of the proposed research are: 1) to explore the usefulness of the cardiac microdialysis technique for the sampling of intramyocardial interstitial fluid (ISF) and measurement of regional coronary blood flow (CBF); and 2) to use the cardiac microdialysis technique to study the cardiac effects of adenosine. In the proposed experiments, cardiac microdialysis, used to determine ISF levels of adenosine, inosine, hypoxanthine, xanthine, uric acid, and lactate, would be utilized in conjunction with standard techniques for the measurement of regional ventricular function and metabolism to perform four series of experiments. Series 1 would assess the validity, applicability, and limitations of the cardiac microdialysis technique. The experiments of Series 2 would take advantage of the capability of the cardiac microdialysis technique to deliver pharmacological agents locally to the tissue surrounding the microdialysis probe, using this approach to study adenosine metabolism and action. In Series 3, cardiac microdialysis would be used to investigate changes in regional ISF purine metabolites and lactate would be correlated to changes in local CBF and regional ventricular function during hypoxia, inotropic stimulation, and graded reductions in coronary artery perfusion pressure. In Series 4, cardiac microdialysis would be used to investigate myocardial ischemia, focusing on interventions which alter endogenous adenosine levels during ischemia.