CARDIAC MICRODIALYSIS, ADENOSINE, AND CARDIAC FUNCTION

描述(改编自申请人的摘要)： 建议的研究是：1)探索心脏的有用性 微透析技术在心肌间质取样中的应用 液体(ISF)和局部冠状动脉血流量(CBF)的测量；以及2) 应用心脏微透析技术研究丹参对心脏的影响 腺苷。在拟议的实验中，心脏微透析用于 测定腺苷、肌苷、次黄嘌呤、黄嘌呤、尿酸的ISF水平 酸和乳酸将与标准的 节段性心功能和心功能的测量技术 进行四个系列的新陈代谢实验。系列1将评估 心脏微透析的有效性、适用性和局限性 技术。系列2的实验将利用 心脏微透析技术的传递能力 微透析周围组织的局部药剂 探头，用这种方法研究腺苷的代谢和作用。在……里面 系列3，心脏微透析将被用来研究 局部ISF嘌呤代谢物和乳酸将与变化相关 低氧、变力时局部脑血流量和局部心功能的变化 刺激，并逐步降低冠脉灌注压。 在系列4中，心脏微透析将被用于研究心肌 缺血，重点是改变内源性腺苷水平的干预措施 在缺血期间。

DESCRIPTION (adapted from applicant's abstract): The objectives of the proposed research are: 1) to explore the usefulness of the cardiac microdialysis technique for the sampling of intramyocardial interstitial fluid (ISF) and measurement of regional coronary blood flow (CBF); and 2) to use the cardiac microdialysis technique to study the cardiac effects of adenosine. In the proposed experiments, cardiac microdialysis, used to determine ISF levels of adenosine, inosine, hypoxanthine, xanthine, uric acid, and lactate, would be utilized in conjunction with standard techniques for the measurement of regional ventricular function and metabolism to perform four series of experiments. Series 1 would assess the validity, applicability, and limitations of the cardiac microdialysis technique. The experiments of Series 2 would take advantage of the capability of the cardiac microdialysis technique to deliver pharmacological agents locally to the tissue surrounding the microdialysis probe, using this approach to study adenosine metabolism and action. In Series 3, cardiac microdialysis would be used to investigate changes in regional ISF purine metabolites and lactate would be correlated to changes in local CBF and regional ventricular function during hypoxia, inotropic stimulation, and graded reductions in coronary artery perfusion pressure. In Series 4, cardiac microdialysis would be used to investigate myocardial ischemia, focusing on interventions which alter endogenous adenosine levels during ischemia.