METABOLIC ADAPTATIONS TO REPETITIVE MYOCARDIAL ISCHEMIA

重复性心肌缺血的代谢适应

• 批准号: 2879389
• 负责人: David G Van Wylen
• 金额: $ 10.59万
• 依托单位: ST. OLAF COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2879389
• 关键词: adenosine; adenosine deaminase; adenosine kinase; cytoprotection; interstitial; laboratory rabbit; microdialysis; myocardial ischemia /hypoxia; purine /pyrimidine metabolism; reperfusion

DESCRIPTION: (Adapted from the Investigator's Abstract) Preconditioning induced by repeated brief myocardial ischemia each separated by another brief period of reperfusion protects the myocardium from subsequent ischemia reperfusion injury. One of beneficial consequences of preconditioning is progressive preservation of high energy phosphate compounds which is reflected by the progressively greater attenuation of the increase in interstitial fluid (ISF) purine metabolites. The proposed research will examine the threshold of the attenuated purine metabolite accumulation by addressing two Specific Aims: 1) by determining the minimum time and degree of ischemia necessary to induce attenuated purine metabolite accumulation during subsequent global ischemia and 2) by determining the effect of adenosine kinase or adenosine deaminase inhibition on ISF purine metabolite accumulation and by studying the rate of adenosine formation during preconditioning.

描述：（改编自研究者摘要）预处理诱导 反复短暂的心肌缺血，每一次都被另一个短暂的 再灌注保护心肌免受随后的缺血再灌注 损伤预处理的有益结果之一是渐进的 高能量磷酸盐化合物的保存， 间质液（ISF）增加的衰减逐渐增大 嘌呤代谢物。拟议的研究将审查的门槛， 通过解决两个特定目的来减弱嘌呤代谢物的蓄积：1） 通过确定诱导缺血所需的最小时间和程度， 在随后的全脑缺血期间减弱嘌呤代谢物的积累， 2)通过测定腺苷激酶或腺苷脱氨酶 抑制ISF嘌呤代谢产物的积累，并通过研究 预处理过程中腺苷的形成。