PARASAGITTAL ORGANIZATION OF CEREBELLAR CORTEX

小脑皮质的旁矢状组织

• 批准号: 3396955
• 负责人: LEONARD M EISENMAN
• 金额: $ 12.96万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-12-19 至 1994-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3396955
• 关键词: brain mapping; brain stem; central neural pathway /tract; cerebellar Purkinje cell; cerebellar cortex; cerebellum; electrophysiology; histochemistry /cytochemistry; immunocytochemistry; innervation; laboratory rat; monoclonal antibody; mossy fiber; neuroanatomy; neuromuscular system; olivary body

The parasagittal organization of the cerebellar cortex has been demonstrated using numerous methodologies, including morphological, biochemical and physiological approaches. These studies, in numerous species, illustrate the apparent commonality of this organizational schema across species and allude to its importance in the function of the cerebellum in the control, coordination and execution of motor behavior. It is essential to analyze these patterns in the same animal in order to determine whether there is an underlying unitary organizational schema to the cerebellum. Recent studies demonstrate that the climbing fiber afferent system, which originates from the inferior olivary complex, share common boundaries with the distribution of the Purkinje cell specific antigen Zebrin 1. Additional studies in the mouse demonstrate identical parasagittal compartments for the Zebrin 1 antigen and the hydrolytic enzyme 5'-nucleotidase. These studies suggest a common compartmentation for these three different features of cerebellar heterogeneity. In this application, we propose to build on these findings and continue our analysis of parasagittal compartmentation by examining the organization of the other major afferent system, mossy fibers, and the cerebellar cortical efferents. Toward this end the following studies will be conducted in the rodent. (1) Analyze the topographic relationship between mossy fiber afferents to the cerebellar cortex. Such projections from the spinal cord, lateral reticular nucleus, basilar pontine nucleus, vestibular nuclei, external cuneate nucleus and trigeminal nuclei will be analyzed utilizing a double anterograde labeling paradigm. (2) Compare the topographic organization of these mossy fiber projections to the distribution of the Zebrin 1 antigen. This will be accomplished by combined anterograde tracer and immunohistological analysis within the same animal. (3) Analyze the efferent projection of Purkinje cells in the cerebellar cortex, as determined by retrograde tracer analysis, and compare this with the distribution of the Zebrin 1 antigen. These studies will provide essential data which will significantly increase our understanding of cerebellar heterogeneity and thereby help test the hypothesis of a common blueprint for these heterogeneities. The ultimate goal being to develop a more complete understanding of the structural and functional organization of the cerebellum and its influence on normal motor behavior, and abnormal motor behaviors which result from cerebellar disease.

小脑皮质的旁脑组织已经被 使用多种方法，包括形态学， 生物化学和生理学方法。 这些研究，在许多 物种，说明这种组织的明显共性 跨物种的模式，并暗示其在功能的重要性， 小脑在运动控制、协调和执行中的作用 行为 在同一种动物中分析这些模式是至关重要的 为了确定是否存在潜在的单一 组织结构模式到小脑。 最近的研究表明 攀爬纤维传入系统，起源于 下橄榄复合体，与分布有共同边界 浦肯野细胞特异性抗原ZE 1其他研究 小鼠表现出与Zeolus相同的旁隔室， 1抗原和水解酶5 '-核苷酸酶。 这些研究 这三个不同的特征提出了一个共同的划分， 小脑异质性在本申请中，我们建议在 这些研究结果，并继续我们的分析parasystrophy 通过审查其他主要组织的组织来划分 传入系统、苔藓纤维和小脑皮质传出。 为此，将在啮齿动物中进行以下研究。 (1)分析苔藓纤维传入纤维之间的地形图关系 到小脑皮质 脊髓的这种突起， 外侧网状核，基底脑桥核，前庭核， 将分析外侧楔束核和三叉神经核 利用双顺行标记范例。(2)比较 这些苔藓纤维投射到 Zeald 1抗原的分布。 这将通过 结合顺行示踪剂和免疫组织化学分析， 同样的动物。(3)分析浦肯野细胞的传出投射 小脑皮质，通过逆行示踪分析确定，和 将其与Zeald 1抗原的分布进行比较。 这些 研究将提供必要的数据， 我们对小脑异质性的理解，从而帮助测试 这些异质性的共同蓝图的假设。 的 最终目标是更全面地了解 小脑的结构和功能组织及其 对正常运动行为和异常运动行为的影响， 小脑疾病的结果。