CHLORIDE TRANSPORT IN NERVE AND MUSCLE

神经和肌肉中的氯离子转运

基本信息

  • 批准号:
    2262374
  • 负责人:
  • 金额:
    $ 30.27万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1977
  • 资助国家:
    美国
  • 起止时间:
    1977-09-01 至 1995-08-31
  • 项目状态:
    已结题

项目摘要

This is a proposal to study two different chloride transport mechanisms; both of which appear to be widely distributed in animal cells. One mechanism uses movements of C1, Na and HCO3 to extrude or neutralize intracellular acidity. In this proposal we will examine how this system is activated by acidic intracellular pH and how it changes from an apparent C1/C1 exchange mechanism to a more complicated Na and HCP3 - dependent acid extruder. Our central hypothesis is that ATP-dependent phosphorylation plays a key role in these events. We will perform detailed studies of ion transport to obtain quantitative kinetic information about this transport mechanism. The second chloride transport mechanism to be studied is the Na, K, Cl co-transporter. This transporter also requires ATP but presumably not for energetic or thermodynamic reasons. We believe phosphorylation is also involved for the regulation of activity by this transporter. In both transporters we will study fundamental transport properties as well as the effects of agents to stimulate and/or inhibit protein kinases and protein phosphatases in an effort to substantiate our hypothesis of transport activation via protein phosphorylation. These studies will be conducted on giant cells (squid giant axon and barnacle giant muscle fibers). Their large size allows us to use the technique of intracellular dialysis to control both intra-and extracellular contents. The method permits the measurement of unidirectional ion fluxes by these chloride transport mechanisms, we will use radioactive-isotope-labelled ligands to attempt to label and partially identify the membrane protein(s) involved in the transport processes.
这是研究两种不同的氯离子输运机制的建议;

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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JOHN M RUSSELL其他文献

JOHN M RUSSELL的其他文献

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{{ truncateString('JOHN M RUSSELL', 18)}}的其他基金

REGULATION OF THE SQUID AXON NA, K, C1 COTRANSPORTER
鱿鱼轴突 NA、K、C1 协同转运蛋白的调节
  • 批准号:
    6639719
  • 财政年份:
    2000
  • 资助金额:
    $ 30.27万
  • 项目类别:
REGULATION OF THE SQUID AXON NA, K, CL COTRANSPORTER
鱿鱼轴 NA、K、CL 协同转运蛋白的调节
  • 批准号:
    6038332
  • 财政年份:
    2000
  • 资助金额:
    $ 30.27万
  • 项目类别:
REGULATION OF THE SQUID AXON NA, K, C1 COTRANSPORTER
鱿鱼轴突 NA、K、C1 协同转运蛋白的调节
  • 批准号:
    6394386
  • 财政年份:
    2000
  • 资助金额:
    $ 30.27万
  • 项目类别:
REGULATION OF THE SQUID AXON NA, K, C1 COTRANSPORTER
鱿鱼轴突 NA、K、C1 协同转运蛋白的调节
  • 批准号:
    6540369
  • 财政年份:
    2000
  • 资助金额:
    $ 30.27万
  • 项目类别:
ION TRANSPORT IN NERVE AND FIBROBLASTS
神经和成纤维细胞中的离子传输
  • 批准号:
    6213630
  • 财政年份:
    1998
  • 资助金额:
    $ 30.27万
  • 项目类别:
ION TRANSPORT IN NERVE AND FIBROBLASTS
神经和成纤维细胞中的离子传输
  • 批准号:
    6033051
  • 财政年份:
    1998
  • 资助金额:
    $ 30.27万
  • 项目类别:
ION TRANSPORT IN NERVE AND FIBROBLASTS
神经和成纤维细胞中的离子传输
  • 批准号:
    2460487
  • 财政年份:
    1977
  • 资助金额:
    $ 30.27万
  • 项目类别:
ION TRANSPORT IN NERVE AND FIBROBLASTS
神经和成纤维细胞中的离子传输
  • 批准号:
    2750804
  • 财政年份:
    1977
  • 资助金额:
    $ 30.27万
  • 项目类别:
CHLORIDE TRANSPORT IN NERVE AND MUSCLE
神经和肌肉中的氯离子转运
  • 批准号:
    3394667
  • 财政年份:
    1977
  • 资助金额:
    $ 30.27万
  • 项目类别:
ION TRANSPORT IN NERVE AND FIBROBLASTS
神经和成纤维细胞中的离子传输
  • 批准号:
    2262375
  • 财政年份:
    1977
  • 资助金额:
    $ 30.27万
  • 项目类别:
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