CHLORIDE TRANSPORT IN NERVE AND MUSCLE
神经和肌肉中的氯离子转运
基本信息
- 批准号:2262374
- 负责人:
- 金额:$ 30.27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1977
- 资助国家:美国
- 起止时间:1977-09-01 至 1995-08-31
- 项目状态:已结题
- 来源:
- 关键词:Cirripedia acidity /alkalinity adenosinetriphosphatase alternatives to animals in research axon carbonates chlorine ligands membrane permeability membrane proteins membrane transport proteins microelectrodes muscle cells phosphoprotein phosphatase phosphorus phosphorylation potassium protein kinase radionuclides radiotracer saltwater environment sodium squid stoichiometry
项目摘要
This is a proposal to study two different chloride transport mechanisms;
both of which appear to be widely distributed in animal cells. One
mechanism uses movements of C1, Na and HCO3 to extrude or neutralize
intracellular acidity. In this proposal we will examine how this system is
activated by acidic intracellular pH and how it changes from an apparent
C1/C1 exchange mechanism to a more complicated Na and HCP3 - dependent acid
extruder. Our central hypothesis is that ATP-dependent phosphorylation
plays a key role in these events. We will perform detailed studies of ion
transport to obtain quantitative kinetic information about this transport
mechanism. The second chloride transport mechanism to be studied is the
Na, K, Cl co-transporter. This transporter also requires ATP but
presumably not for energetic or thermodynamic reasons. We believe
phosphorylation is also involved for the regulation of activity by this
transporter. In both transporters we will study fundamental transport
properties as well as the effects of agents to stimulate and/or inhibit
protein kinases and protein phosphatases in an effort to substantiate our
hypothesis of transport activation via protein phosphorylation.
These studies will be conducted on giant cells (squid giant axon and
barnacle giant muscle fibers). Their large size allows us to use the
technique of intracellular dialysis to control both intra-and extracellular
contents. The method permits the measurement of unidirectional ion fluxes
by these chloride transport mechanisms, we will use
radioactive-isotope-labelled ligands to attempt to label and partially
identify the membrane protein(s) involved in the transport processes.
这是研究两种不同的氯离子输运机制的建议;
项目成果
期刊论文数量(0)
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科研奖励数量(0)
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专利数量(0)
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JOHN M RUSSELL其他文献
JOHN M RUSSELL的其他文献
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{{ truncateString('JOHN M RUSSELL', 18)}}的其他基金
REGULATION OF THE SQUID AXON NA, K, C1 COTRANSPORTER
鱿鱼轴突 NA、K、C1 协同转运蛋白的调节
- 批准号:
6639719 - 财政年份:2000
- 资助金额:
$ 30.27万 - 项目类别:
REGULATION OF THE SQUID AXON NA, K, CL COTRANSPORTER
鱿鱼轴 NA、K、CL 协同转运蛋白的调节
- 批准号:
6038332 - 财政年份:2000
- 资助金额:
$ 30.27万 - 项目类别:
REGULATION OF THE SQUID AXON NA, K, C1 COTRANSPORTER
鱿鱼轴突 NA、K、C1 协同转运蛋白的调节
- 批准号:
6394386 - 财政年份:2000
- 资助金额:
$ 30.27万 - 项目类别:
REGULATION OF THE SQUID AXON NA, K, C1 COTRANSPORTER
鱿鱼轴突 NA、K、C1 协同转运蛋白的调节
- 批准号:
6540369 - 财政年份:2000
- 资助金额:
$ 30.27万 - 项目类别: