H & D EXCHANGE OF INSULINS

新的蛋白质和肽类药物的不断涌现 需要开发快速和灵敏的方法， 检查生物技术批次之间和批次内的一致性 药品，以确保产品质量。 报告的持续工作 去年，我们研究了胰岛素浓度对 两种胰岛素（重组人胰岛素和Lis/Pro）的氢交换 为了合理化数量上的巨大差异， 对其他突变体进行了额外的工作， 以确定结构的微小变化是否会导致 引起氢交换数量的巨大变化。

The increasing emergence of new protein and peptide-based drugs makes necessary the development of rapid and sensitive methods to check consistency between and within batches of biotechnology pharmaceuticals to ensure product quality. Continuing work reported last year, we investigated the effects of insulin concentration on the hydrogen exchange of the two insulins, recombinant human and Lis/Pro in an attempt to rationalize the large difference in numbers of hydrogens exchanged .Additional work was performed on other mutant insulins to determine if small changes in the structure could give rise to large changes in the numbers of hydrogens exchanged.