Microarray Center for Research on the Nervous System

描述（由申请人提供）：微阵列研究中心 遗传医学中心，儿童？美国国家医疗中心，华盛顿特区 提供了所附的建议，其中NINDS/NIMH微阵列的概述 中心（MC）的表达谱进行了描述。协助实验 将提供设计、数据生成和数据解释。是 重要的是要认识到，儿童？的微阵列中心已经实现了 NHLBI，NEI和许多其他调查人员的这些功能，而且，事实上， 完善的实验方案、网站和创新的 生物信息学方法允许相对高通量的生物信息学方法， 费用低（由于与其他赠款和方案分摊费用）。简单地说，MC 是独一无二的，因为我们已经建立了一个表达分析中心， 通过1）NHLBI PGA大规模表达奖 分析（全国仅有的11个之一）和NEI内部合同的cDNA 建设，2）成为Affyoung技术的“卓越中心”， 3)开发一个高度集成的阵列网站 （microarray.cnmcresearch.org），用于教程和数据传播，4） 开发了第一个基于Web的Oracle数据查询算法 库，5）精通cDNA和亲和素技术，6） 已经进行了超过975次的Affymble阵列实验，7）MC的操作 作为一家拥有常驻基础设施的企业，8）协助50多名来访者 研究人员与阵列实验迄今为止，和9）有居民的科学 实验设计协助的专业知识。在本次活动中，我们建议 建立一个NINDS/NIMH微阵列中心，在 现有儿童？微阵列中心。大部分拟议结构和 MC的功能是基于我们以前的经验， NHLBI资助的赠款。MC的目标包括：1）基于Web的协议 教程和提交，2）通过实验方案进行审查和修订 具有丰富神经科学经验的委员会（EPC），3） 制造商驱动的收费机制（3级补贴），4）数据 代，5）Oracle仓库，自动定时发布给公众 通过我们网站和NCBI的网络访问，6）我们数据库的网络查询，5） 数据分析教程和帮助，以及6）开发自定义Affytechnology 神经芯片使用我们的下游大型数据结构和我们的“中心 卓越”的地位作为机制，开发一种独特的，低成本的试剂， 社区虽然我们擅长斑点cDNA阵列和亲和素， 阵列，我们计划提供Affyscore股票芯片作为主要的数据生成 NINDS/NIMH研究人员的平台。这是因为， 可移植性和数据库结果的能力，允许所有 调查人员使用所有其他调查人员的结果。还 数据库和Web访问Oracle/LIMS中的核心数据库结构 Affyoung平台正变得相当成熟，而这样的数据库和Web 用点样cDNA阵列的访问相当不成熟。我们已经打印 并使用cDNA阵列（特别是小鼠BMAP）进行了大型实验 该平台将提供给NINDS/NIMH资助的研究人员， 有限的可选服务，可用性，即使大多数基因 BMAP集合中的所有数据也在MG_U74阵列上（超过74%）。

DESCRIPTION (provided by applicant): The Microarray Center of the Research Center of Genetic Medicine, Children?s National Medical Center, Washington DC offers the attached proposal, wherein an outline of a NINDS/NIMH Microarray Center (MC) for expression profiling is described. Assistance with experimental design, data generation, and data interpretation will be provided. It is important to recognize that the Children?s Microarray Center already fulfills such functions for NHLBI, NEI, and many other investigators, and, indeed, has well-established experimental protocols, web sites, and innovative bioinformatics methods which permit a high degree of throughput at relatively low cost (due to cost sharing with other grants and programs). Briefly, the MC is unique because we have built an expression profiling Center and amassed significant expertise through 1) an NHLBI PGA award for large-scale expression profiling (one of only 11 nationally) and an NEI intramural contract for cDNA construction, 2) becoming a "Center of Excellence" for Affymetrix technology, 3) development of a highly integrated array web site (microarray.cnmcresearch.org) for tutorials and data dissemination, 4) development of the first web-based query algorithm of our Oracle data repository, 5) being well-versed in both cDNA and Affymetrix technology, 6) having performed over 975 Affymetrix array experiments, 7) operation of the MC as a business with resident infrastructure, 8) assisting over 50 visiting investigators with array experiments to date, and 9) having resident scientific expertise for experimental design assistance. In this offering, we propose to establish a NINDS/NIMH Microarray Center for expression profiling within the existing Children?s Microarray Center. Much of the proposed structure and function of the MC is based upon our previous experience with a similar NHLBI-funded grant. The goals for the MC include: 1) web-based protocol tutorials and submission, 2) review and revisions by an experimental protocol committee (EPC) which has extensive neuroscience experience, 3) investigator-driven charge-back mechanisms (3 levels of subsidization), 4) data generation, 5) Oracle warehousing with automated timed release to the public via web-access at our site and NCBI, 6) web queries of our data repository, 5) data analysis tutorials and assistance, and 6) development of custom Affymetrix NeuroChips using our downstream large data structure and our "Center of Excellence" status as mechanisms to develop a unique, low-cost reagent for the community. While we are adept at both spotted cDNA arrays and Affymetrix arrays, we plan to offer Affymetrix stock chips as the primary data generation platform for NINDS/NIMH investigators. This is because of the inherent transportability and the ability to database results, allowing all investigators to use the results of all other investigators. Also, the databasing and web access to kernal database structures in the Oracle/LIMS Affymetrix platform is becoming quite mature, while such databasing and web access is considerably less mature with spotted cDNA arrays. We have printed and conducted large experiments with cDNA arrays (particularly the murine BMAP set), and this platform will be available to NINDS/NIMH-funded investigators as an optional service at limited, availability, even though the majority of genes in the BMAP collection are also on the MG_U74 array (more than 74%).