Protein Expression in Tissue of Ovarian Cancer Patients

卵巢癌患者组织中的蛋白质表达

• 批准号: 6695492
• 负责人: Alice Whittemore
• 金额: $ 8万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-20 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6695492
• 关键词: biomarker; cancer registry /resource; clinical research; female; gene expression; histopathology; human tissue; ovary neoplasms; proteins; sample collection; tissue resource /registry

DESCRIPTION (provided by applicant): We have gathered epidemiological data and blood from a population-based sample of epithelial ovarian cancer patients in Northern California. Among these patients, 667 (94 percent) have provided permission to obtain samples of their paraffin-embedded ovarian tissue. For this pilot study, we request funds to collect these tissue samples and to analyze them for specific protein expression patterns, as they relate to particular characteristics of the patients and their tumors. Specifically, this pilot study aims to: 1) determine expression levels of 10 proteins considered to be potentially important in the pathogenesis of ovarian cancer; and 2) compare expression levels across subgroups defined by patient characteristics, histopathological characteristics of tumor, and combinations of patient and histopathological characteristics. Our long-term aims (to be accomplished under future funding) are to: i) study many additional proteins involved in the hormonal and cell-cycle pathways represented by the current sample of proteins; and ii) relate protein expression levels to ovarian cancer survival and to germline polymorphisms that are associated with ovarian cancer risk. Detection of protein expression variation across patient and tumor characteristics should help to develop and evaluate potential screening tools for early detection and new treatment strategies. Such detection also may advance our understanding of the pathogenesis of ovarian cancer.

描述(由申请人提供)： 我们从北加州上皮性卵巢癌患者的人群样本中收集了流行病学数据和血液。在这些患者中，667人(94%)提供了获取石蜡包埋卵巢组织样本的许可。对于这项初步研究，我们要求资金收集这些组织样本，并分析它们的特定蛋白质表达模式，因为它们与患者及其肿瘤的特定特征有关。具体地说，这项先导性研究的目的是：1)确定被认为在卵巢癌发病机制中潜在重要的10种蛋白质的表达水平；以及2)比较由患者特征、肿瘤的组织病理学特征以及患者和组织病理学特征的组合定义的不同亚组的表达水平。我们的长期目标(将在未来的资助下实现)是：i)研究当前蛋白质样本所代表的荷尔蒙和细胞周期途径中的许多额外蛋白质；ii)将蛋白质表达水平与卵巢癌生存和与卵巢癌风险相关的生殖系多态联系起来。检测患者和肿瘤特征之间的蛋白表达差异，有助于开发和评估潜在的筛查工具，用于早期检测和新的治疗策略。这样的检测也可能促进我们对卵巢癌发病机制的了解。