Regulation of Angiopoietin mRNA Stability

血管生成素 mRNA 稳定性的调节

• 批准号: 6599782
• 负责人: JOHN VOGEL
• 金额: $ 11.34万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6599782
• 关键词: RNA binding protein; RNA biosynthesis; affinity chromatography; angiogenesis; angiopoietins; animal genetic material tag; biological signal transduction; cell growth regulation; complementary DNA; enzyme activity; gel mobility shift assay; genetic regulatory element; human genetic material tag; messenger RNA; phosphatidylinositol 3 kinase; polymerase chain reaction; protein binding; protein purification; protein sequence; transcription factor; vascular endothelium; vascular smooth muscle; western blottings

DESCRIPTION (provided by applicant): A five year training program of integrated didactic and laboratory experiences to facilitate the development of an academic career involving molecular biology based research. The principle investigator has successfully completed a residency in Anatomic and Clinical Pathology and is currently in a two-year Molecular Genetic Pathology Fellowship. To build toward the goal of obtaining a tenure track position in an academic setting, and to combine a career in both basic research and academic medicine, the choice was made to obtain education and experience in molecular biological research involving angiogenesis and its role in diseases of the eye. This goal will be accomplished through a challenging research proposal focused on signal transduction pathways associated with Angiopoietin-2 (Ang2) mRNA stability. Ang2 is critically involved in blood vessel stability, and is involved in ocular neovascularization. Preliminary data indicate that Ang2 is significantly regulated post-transcriptionally, and that phosphoinositide-3-kinase (PI3K) activity plays a critical role in Ang2 mRNA stability. We will identify cis-acting elements in Ang2 mRNA and the associated trans-acting factors involved in PI3K-mediated Ang2 mRNA stability. Drs. Anderson (Mentor - Chair of the Department of Cell Biology) and Howard (Co-Mentor - Associate Professor of Cell Biology) have extensive research experience in the fields of eye disease (including the role of PI3K in ocular disease) and angiogenesis (including the regulation angiopoietins), respectively. The University of Oklahoma Health Sciences Center is strongly committed to ocular research through the Dean McGee Eye Institute. The facilities available to the principal investigator provide the modem equipment necessary for this level of molecular-based research.

描述（由申请人提供）：一个为期五年的综合教学和实验室经验的培训计划，以促进涉及分子生物学研究的学术生涯的发展。主要研究者已成功完成了解剖和临床病理学的住院医师培训，目前正在进行为期两年的分子遗传病理学研究。为了实现在学术环境中获得终身职位的目标，并将基础研究和学术医学的职业生涯联合收割机结合起来，选择在涉及血管生成及其在眼部疾病中的作用的分子生物学研究中获得教育和经验。这一目标将通过一项具有挑战性的研究计划来实现，该计划侧重于与血管生成素-2（Ang 2）mRNA稳定性相关的信号转导途径。Ang 2与血管稳定性密切相关，并参与眼部新生血管形成。初步数据表明Ang 2在转录后受到显著调节，并且磷酸肌醇-3-激酶（PI 3 K）活性在Ang 2 mRNA稳定性中起关键作用。我们将鉴定Ang 2 mRNA中的顺式作用元件和参与PI 3 K介导的Ang 2 mRNA稳定性的相关反式作用因子。安德森博士（导师-细胞生物学系主任）和霍华德博士（联合导师-细胞生物学副教授）分别在眼科疾病（包括PI 3 K在眼科疾病中的作用）和血管生成（包括血管生成素的调节）领域拥有丰富的研究经验。俄克拉荷马州大学健康科学中心通过Dean McGee眼科研究所致力于眼科研究。主要研究者可利用的设施提供了这种水平的分子基础研究所需的现代化设备。