ACTIVATED ENDOTHELIAL ADHESIVITY IN SC VASOOCCLUSION

SC 血管闭塞中的活化内皮粘附力

• 批准号: 6617851
• 负责人: Stephen H. Embury
• 金额: $ 31.97万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-27 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6617851
• 关键词: blood vessel occlusion; cell adhesion; cell adhesion molecules; cell line; clinical research; disease /disorder model; erythrocytes; fluorescence microscopy; gene targeting; genetically modified animals; human subject; intravital microscopy; laboratory mouse; sickle cell anemia; thrombin receptor; vascular endothelium

DESCRIPTION: (Adapted from investigator's abstract) This proposal focuses on the adhesogenic responses of endothelial cells to agonists germane to sickle cell disease. The specific aims are to test the following hypotheses: 1) that thrombin and hypoxia (I/R) enhance EC adhesivity for SS RB; 2) that specific EC adhesive molecules account for increased adhesivity; and 3) that identifiable cellular mechanisms account for the increased adhesivity. Aims 1 and 2 will be studied in vitro and, using a mouse model of sickle cell disease, in vivo. The singular focus on activated endothelial cell adhesivity provides an innovative perspective to this proposal. The availability of a transgenic knockout sickle cell mouse model highly concordant with the human disease and of a state-of-the-art intravital microscopy system provide an exceptional opportunity to verify in vivo the importance of in vitro adherence phenomena. These experiments are anticipated to enhance our understanding of the molecular mechanisms of sickle erythrocyte-endothelial adherence, improve our grasp of the pathophysiology of sickle cell vasoocclusion, and expand our knowledge of endothelial cell biology. They may provide the basis for future therapeutic interventions.

描述：（改编自研究者摘要）本提案的重点是

项目成果

L-glutamine therapy reduces endothelial adhesion of sickle red blood cells to human umbilical vein endothelial cells.

• DOI: 10.1186/1471-2326-5-4
• 发表时间: 2005-07-25
• 期刊: BMC blood disorders
• 作者: Niihara, Yutaka;Matsui, Neil M;Tanaka, Kouichi R
• 通讯作者: Tanaka, Kouichi R