Novel Raman Method for Molecular Analysis of Tissue

描述（由申请人提供）：拟议的项目是开发一种振动光谱系统，该系统可以在强荧光存在下获得良好的信噪比（S/N）拉曼光谱。对于许多组织样品，强的天然荧光的存在禁止使用拉曼或共振拉曼光谱技术进行分子水平分析。基于可调谐二极管激光技术和阵列检测方案的新组合，物理科学公司。(PSI)发明了一种能够在存在强的叠加荧光的情况下有效地提取拉曼信号的技术。荧光抑制的幅度可以高达100 dB或更高。在第一阶段，该系统将从目前的概念验证设置进行优化。该系统随后将用于对组织样本进行拉曼光谱分析。PSI将证明，我们的系统有能力获得优良的S/N比拉曼光谱从各种组织样品中存在的强烈叠加荧光。这将最终促进体外和体内分子疾病诊断使用拉曼光谱。使用这种技术，它也将被证明，共振拉曼光谱可以在强荧光组织样品进行分析痕量分子。第二阶段的目标是建立和测试一个原型系统，用于研究和临床应用。

DESCRIPTION (provided by applicant): The proposed project is to develop a vibrational spectroscopic system that can obtain good signal to noise ratio (S/N) Raman spectra in the presence of strong fluorescence. For many tissue samples the presence of strong native fluorescence prohibits molecular level analysis using Raman or resonance Raman spectroscopic techniques. Based on a novel combination of tunable diode laser technology and an array detection scheme, Physical Sciences Inc. (PSI) has devised a technology that is capable of efficiently extracting Raman signals in the presence of strong overlying fluorescence. The magnitude of fluorescence rejection can be as high as 100 dB or more. In the Phase I period, the system will be optimized from the current proof-of-concept setup. The system will be subsequently used to perform Raman spectroscopy analysis of tissue samples. PSI will demonstrate that our system has the capability to obtain excellent S/N ratio Raman spectra from a variety of tissue samples in the presence of strong overlying fluorescence. This will ultimately facilitate in vitro and in vivo molecular disease diagnosis using Raman spectroscopy. Using this technique, it will also be demonstrated that Resonance Raman spectroscopy can be performed in strongly fluorescent tissue samples to analyze trace molecules. The Phase II objective will be building and testing a prototype system for research and clinical applications.