Array microscope image-acquisition system

阵列显微镜图像采集系统

• 批准号: 6690912
• 负责人: Pixuan Zhou
• 金额: $ 10.7万
• 依托单位: DMETRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2005-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6690912
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; biotechnology; digital imaging; high throughput technology; histopathology; image processing; microscopy; miniature biomedical equipment; optics; tissue /cell preparation

DESCRIPTION (provided by applicant): DMetrix is developing an innovative array-microscope slide scanner to generate virtual slides in histopathology. The innovation consists of the use of an array of miniaturized objectives, each measuring approximately 2 mm in diameter and 10 mm in length, slightly staggered in the array, to produce a single, seamless sweep of the microscope slide. The combination of the array microscope optics and the image acquisition system proposed here will allow very rapid scanning, requiring less than 12 seconds for a full microscope slide of 20 mm x 50 mm, at diffraction-limited sampling, The proposed image-acquisition system consists of an optimized, high-speed CMOS image sensor operating at 60 MHz with eight (8) parallel outputs and the accompanying high-speed electronics. The CMOS image sensor is optimized to match the format of the array-microscope optics. The proposed highspeed electronics will be designed to control the image sensor, digitize the image sensor's analog outputs, process the image data in real-time, and compress the image data prior to storage on a host personal computer (PC). The array-microscope virtual-slide scanner will allow digitization of slides at rates 50 times faster than conventional single-optical-axis optical systems. Applications of such a virtual slide scanner are to be found in histopathology, telepathology, pathology education, and pharmaceutical research.

描述（由申请人提供）： DMeetron正在开发一种创新的阵列显微镜载玻片扫描仪，以生成组织病理学中的虚拟载玻片。该创新包括使用微型物镜阵列，每个直径约为2 mm，长度为10 mm，在阵列中略微交错，以产生显微镜载玻片的单一无缝扫描。本文提出的阵列显微镜光学器件和图像采集系统的组合将允许非常快速的扫描，对于20 mm x 50 mm的完整显微镜载玻片，在衍射限制的采样下需要不到12秒。高速CMOS图像传感器，工作频率为60 MHz，具有8个并行输出和随附的高速电子器件。CMOS图像传感器经过优化以匹配阵列显微镜光学器件的格式。所提出的高速电子设备将被设计成控制图像传感器，对图像传感器的模拟输出进行采样，实时处理图像数据，并在将图像数据存储在主机个人计算机（PC）上之前对其进行压缩。阵列显微镜虚拟载玻片扫描仪将允许数字化的速度比传统的单光轴光学系统快50倍。这样的虚拟载玻片扫描仪的应用被发现在组织病理学，远程病理学，病理学教育，和药物研究。