Array microscope image-acquistion system

阵列显微镜图像采集系统

• 批准号: 6994523
• 负责人: Pixuan Zhou
• 金额: $ 81.16万
• 依托单位: DMETRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6994523
• 关键词: Array; microscope; image; acquistion; system

DESCRIPTION (provided by applicant): DMetrix is developing an innovative array-microscope technology to generate virtual slides in histopathology and cytology. The innovation consists of the use of an array of miniaturized objectives, each measuring approximately 2 mm in diameter and 10 mm in length, slightly staggered in the array, to produce a single, seamless sweep of the microscope slide. In Phase I, we completed successfully a preliminary design of a state-of-the-art CMOS image sensor. We were able to establish the feasibility of all specifications laid out in Phase I and found no technical barriers to the fabrication of such an imaging device. Furthermore, we successfully developed an analog-to-digital conversion (ADC) electronics board, designed specifically to interface with the proposed image sensor. All success criteria of Phase I were either met or exceeded. In this Phase II application, we propose to complete the development of a 480 MB/sec image-acquisition system for an array-microscope slide scanner to generate virtual slides in histopathology and cytology. The first Aim is to complete the design and fabrication of the CMOS image sensor. The most distinguishing features of this image sensor are its 1.65-mu m pixels, its large 27-mm-widm format, and its combined output data rate of 480 MB/sec. The impact of the new image sensor on our technology will be profound: a quadrupling of slide scanning speed and a doubling of spatial resolution to reach equivalence with 40X conventional microscope optics. The second Aim is to develop prototype image capture & processing electronics that will achieve real-time JPEG2000 image compression and have the potential for enabling real-time image analysis carried out on sixteen PowerPC processors. DMetrix has extensive experience will all facets of the required effort in Phase II. This experience was gained during the development of our current product, a first-of-its-kind array-microscope scanner. The proposed image-acquisition system will lead to a virtual-slide scanner with applications in histopathology, cytology, telepathology, pathology education, pharmaceutical research, and industrial inspection. To our best knowledge, the resulting virtual-slide scanner will have a minimum 20-fold speed competitive advantage relative to our closest competitor and will double the spatial resolution of our current product to 0.235 mu m sampling.

描述(申请人提供)：DMetrix正在开发一种创新的阵列显微镜技术，以生成组织病理学和细胞学的虚拟幻灯片。这一创新包括使用微型物镜阵列，每个物镜直径约2 mm，长度约10 mm，在阵列中略有交错，以产生单个无缝扫描的显微镜载玻片。在第一阶段，我们成功地完成了最先进的cmos图像传感器的初步设计。我们能够确定第一阶段中列出的所有规格的可行性，并发现制造这种成像设备没有技术障碍。此外，我们还成功开发了一块模数转换(ADC)电子板，专门为与所提出的图像传感器接口而设计。第一阶段的所有成功标准都达到或超过了。 在这个第二阶段的应用中，我们计划完成一个480MB/秒的图像采集系统的开发，用于阵列显微镜载玻片扫描仪，以生成组织病理学和细胞学的虚拟载玻片。第一个目标是完成CMOS图像传感器的设计和制造。这款图像传感器的最大特点是1.65微米像素，27毫米宽的大格式，以及480 MB/秒的综合输出数据速率。新的图像传感器将对我们的技术产生深远的影响：载玻片扫描速度翻两番，空间分辨率翻一番，达到与40倍传统显微镜光学系统相当的水平。第二个目标是开发原型图像捕获和处理电子设备，它将实现实时JPEG2000图像压缩，并有可能在16个PowerPC处理器上进行实时图像分析。 DMetrix拥有丰富的经验，将在第二阶段所需的工作的所有方面。这些经验是在我们目前的产品开发过程中获得的，这是同类产品中的第一台阵列显微镜扫描仪。 拟议的图像采集系统将导致一种虚拟幻灯片扫描仪，应用于组织病理学、细胞学、远程病理学、病理学教育、制药研究和工业检查。据我们所知，与我们最接近的竞争对手相比，由此产生的虚拟幻灯片扫描仪将具有至少20倍的速度竞争优势，并将使我们当前产品的空间分辨率翻一番，达到0.235微米采样。