Hyperspectral Microscope for Drusen Phenotyping

用于玻璃疣表型分析的高光谱显微镜

• 批准号: 6693982
• 负责人: Paul W. TRUITT
• 金额: $ 20.68万
• 依托单位: KESTREL CORPORATION
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6693982
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; charge coupled device camera; clinical biomedical equipment; computer program /software; eye disorder diagnosis; fluorescence; human tissue; immunocytochemistry; infrared spectrometry; macular degeneration; macular drusen; medical records; microscopy; optics; phenotype; retinography; tissue resource /registry

DESCRIPTION (provided by applicant): The demonstration of a Fourier-transform hyperspectral microscope for high resolution spectral ex vivo characterization of human retinal tissue is the aim of this project. The spectral reflectance and intrinsic fluorescence nature of normal and pathological retinal tissue must be established without the interfering effects of the intervening ocular media. This motivates the immediate goal of integrating the Fourier-transform imaging spectrometer (FTIS) to a microscope. A hyperspectral microscope may be used in many areas of biological and medical research; tissue classification (normal vs. pathological) and characterization, detection of bacteria, gene identification, chromatin distribution, drug safety and efficacy, molecular imaging, and countless other applications. The Fourier-transform technology provides for a large number of highly resolved spectral bands simultaneously spatial at each pixel in the scene. Since fluorescence intensities are several orders of magnitude less than the reflectance signal, it is necessary to use a spectrometer with the highest possible sensitivity and wavelength selectivity. The FTIS has the advantage over traditional dispersive-based spectrometers in that entrance aperture and spectral resolution are decoupled. Thus, the slit can be increased to allow more light into the system without reducing spectral resolution. Further, because the FTIS is a constant wave number device, it offers improved spectral resolution in the visible to short wave infrared bands for a given detector size over conventional dispersive spectral imagers. Finally, the spatially modulated device collects all spectral data at once, avoiding any issues with spectral registration that otherwise occur. The six-month Phase I project will demonstrate the sensitivity of the hyper-spectral imaging methodology for spectrally phenotyping drusen in age-related macular degeneration (ARMD) eyes. Our approach builds on Kestrel's existing hyperspectral instruments and software, developed through Department of Defense. High spectral (2-4 nm) and spatial resolution images will be collected of histological specimens from two normal donor eyes and two eyes with clinical evidence or pre-mortem diagnosis of ARMD. The integrated system will be calibrated and tested. Illumination requirements for recording fluorescence will be established. A detailed, multi-source data set will be produced consisting of measurements of reflectance and transmission spectra, and intrinsic fluorescence spectra.

描述（由申请人提供）：该项目的目的是人类视网膜组织的高分辨率光谱特征表征的傅立叶转换高光谱显微镜的演示。必须建立正常和病理性视网膜组织的光谱反射率和固有的荧光性质，而无需干预眼培养基的干扰作用。这激发了将傅立叶转换成像光谱仪（FTI）集成到显微镜的直接目标。 高光谱显微镜可以用于生物学和医学研究的许多领域。组织分类（正常与病理）和特征，细菌的检测，基因鉴定，染色质分布，药物安全和功效，分子成像以及无数其他应用。傅立叶变换技术为场景中每个像素的空间同时提供了大量高度分辨的光谱带。由于荧光强度比反射率信号小几个数量级，因此有必要使用具有最高灵敏度和波长选择性的光谱仪。 FTI比传统的基于分散的光谱仪具有优势，在该入口孔径和光谱分辨率被解耦。因此，可以增加缝隙，以使系统中有更多的光线，而无需降低光谱分辨率。此外，由于FTI是一个恒定的波数设备，因此在传统的色散光谱成像器上，它可为给定的检测器尺寸可见到可见的短波红外带提供改进的光谱分辨率。最后，空间调制的设备一次收集所有光谱数据，避免了其他频谱注册问题。六个月的I期项目将证明与年龄相关的黄斑变性（ARMD）眼睛中频谱表型drusen的超光谱成像方法的敏感性。我们的方法以国防部开发的Kestrel现有的高光谱工具和软件为基础。高光谱（2-4 nm）和空间分辨率图像将从两只正常的供体眼中收集组织学标本，两只眼睛具有临床证据或对ARMD的验证前诊断。集成系统将进行校准和测试。将确定记录荧光的照明要求。将产生一个详细的多源数据集，该数据集由反射率和透射光谱的测量以及固有的荧光光谱组成。