Precision Microfluidic Contol for Nanobiotechnology

纳米生物技术的精密微流体控制

• 批准号: 6645624
• 负责人: DAVID WM MARR
• 金额: $ 19.99万
• 依托单位: METAFLUIDICS, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-13 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6645624
• 关键词: bioengineering /biomedical engineering; biotechnology; cell sorting; flow cytometry; fluid flow; fluorescence; microcapsule; microprocessor /microchip; nanotechnology; phenotype; technology /technique development

DESCRIPTION (provided by applicant): An automated microfluidic chip-top flow image cytometer and cell sorter has been developed that is capable of precisely distinguishing and directing both cells and colloidal particles This approach relies on the natural separation maintained by laminar microfluidic streams and the ability of applied fields, specifically optical manipulation techniques, to translate cells and particles between these streams These microdevices have the potential to perform chip-top microbiology more rapidly and with less associated hardware and preparation time than any other techniques currently available Furthermore, the optical actuation approach allows for seamless integration with widely applied fluorescence labeling and detection schemes Single chip-top devices that employ this technology will be capable precisely handling individual cells and microspheres and will be poised to make a considerable contribution to molecular biology on the nanoscale, and therefore molecular therapeutics in the coming decades The specific aims of this proposal include 1) The expansion of the existing cytometry configuration to accommodate multiple sorting fates by using an optical trap to precisely position particles and cells in channels and allowing laminar flows to direct them to the proper outlet channel 2) The integration of fluorescence based detection for rapid discrimination among cell phenotypes, binding events and particle-bound compounds 3) The increase of sorting rates through optimization of detection schemes, an increase in the number of simultaneous traps, and through parallelization of multiple devices upon the same chip 4) The realization of a working prototype capable of handling cells, performing multiple bead-based analyses and rapidly recovering and interpreting the results of these tests

描述（由申请人提供）：已经开发出一种自动化微流体芯片顶部流式图像细胞仪和细胞分选仪，能够精确区分和引导细胞和胶体颗粒。这种方法依赖于层流微流体流维持的自然分离以及应用场的能力，特别是光学操纵技术，在这些流之间平移细胞和颗粒。这些微型设备有潜力 与目前可用的任何其他技术相比，更快地执行芯片顶部微生物学，并且所需的相关硬件和准备时间更少此外，光学驱动方法可以与广泛应用的荧光标记和检测方案无缝集成，采用该技术的单芯片顶部设备将能够精确处理单个细胞和微球，并将为纳米尺度的分子生物学做出巨大贡献，因此 未来几十年的分子疗法 该提案的具体目标包括 1) 通过使用光学陷阱将颗粒和细胞精确定位在通道中并允许层流将它们引导至正确的出口通道，扩展现有的细胞计数配置以适应多种分选结果 2) 集成基于荧光的检测，用于快速区分细胞表型、结合事件和颗粒结合化合物 3) 通过优化检测方案、增加同时捕获的数量以及通过同一芯片上多个设备的并行化来提高分选率 4) 实现能够处理细胞、执行多种基于微珠的分析并快速恢复和解释这些测试结果的工作原型