Precision Microfluidic Contol for Nanobiotechnology

纳米生物技术的精密微流体控制

• 批准号: 6759252
• 负责人: DAVID WM MARR
• 金额: $ 18.1万
• 依托单位: METAFLUIDICS, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-13 至 2005-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6759252
• 关键词: bioengineering /biomedical engineering; biotechnology; cell sorting; flow cytometry; fluid flow; fluorescence; microcapsule; microprocessor /microchip; nanotechnology; phenotype; technology /technique development

DESCRIPTION (provided by applicant): An automated microfluidic chip-top flow image cytometer and cell sorter has been developed that is capable of precisely distinguishing and directing both cells and colloidal particles This approach relies on the natural separation maintained by laminar microfluidic streams and the ability of applied fields, specifically optical manipulation techniques, to translate cells and particles between these streams These microdevices have the potential to perform chip-top microbiology more rapidly and with less associated hardware and preparation time than any other techniques currently available Furthermore, the optical actuation approach allows for seamless integration with widely applied fluorescence labeling and detection schemes Single chip-top devices that employ this technology will be capable precisely handling individual cells and microspheres and will be poised to make a considerable contribution to molecular biology on the nanoscale, and therefore molecular therapeutics in the coming decades The specific aims of this proposal include 1) The expansion of the existing cytometry configuration to accommodate multiple sorting fates by using an optical trap to precisely position particles and cells in channels and allowing laminar flows to direct them to the proper outlet channel 2) The integration of fluorescence based detection for rapid discrimination among cell phenotypes, binding events and particle-bound compounds 3) The increase of sorting rates through optimization of detection schemes, an increase in the number of simultaneous traps, and through parallelization of multiple devices upon the same chip 4) The realization of a working prototype capable of handling cells, performing multiple bead-based analyses and rapidly recovering and interpreting the results of these tests

DESCRIPTION (provided by applicant): An automated microfluidic chip-top flow image cytometer and cell sorter has been developed that is capable of precisely distinguishing and directing both cells and colloidal particles This approach relies on the natural separation maintained by laminar microfluidic streams and the ability of applied fields, specifically optical manipulation techniques, to translate cells and particles between these streams These microdevices have the potential to与当前可用的任何其他技术相比，执行CHIP-TOP微生物学更快，相关的硬件和准备时间较少，光学驱动方法允许与广泛应用的荧光标记和检测单一芯片顶型设备无缝集成，以使该技术能够准确地促进单个细胞和微分球构成，并可以使单个细胞和微地位贡献，并且可以贡献单个细胞和微地位。分子治疗剂在未来几十年中，该提案的具体目的包括 1）现有的细胞仪构型的扩展，通过使用光学陷阱来精确定位粒子和细胞在通道中的位置，并允许层流流将其引导到适当的出口通道，以适应多个分类命运 2）基于荧光检测的整合，以在细胞表型，结合事件和粒子结合化合物之间快速区分 3）通过优化检测方案，同时陷阱的数量增加以及通过同一芯片上多个设备并行化来增加排序率 4）实现能够处理细胞，进行多个基于珠的分析并迅速恢复和解释这些测试结果的工作原型的实现