Magnetic nanoparticle-facilitated spinal cord repair

磁性纳米颗粒促进脊髓修复

• 批准号: 6549739
• 负责人: ALAN A HALPERN
• 金额: $ 11.14万
• 依托单位: ORTHOPEDIC DEVELOPMENT, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-19 至 2004-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6549739
• 关键词: antibody; axon; biomagnetism; bovine serum albumin; growth cones; laboratory rat; magnetic field; microsurgery; nanotechnology; nervous system regeneration; polymers; spinal cord injury; spinal cord surgery; technology /technique development

DESCRIPTION (provided by applicant): An injured mammalian spinal cord will not spontaneously repair itself. The goal of this project is the development of a system for axonal growth and restoration of spinal cord function, in which polymer and anti-Li magnetic nanoparticles are attached to the growth cones of traumatized axons, which are then induced to grow distally along the magnetic flux lines from an external magnetic field. This would represent a unique and patented method that would involve (1) a new surgical technique, (2) an injectable device, and (3) a device to create a precise magnetic field. It has been shown that tension applied to CNS neurons does indeed stimulate directional growth. In previous studies, ODI has shown that BSA-coated particles are phagocytosed by CNS neurons and that the cells can be directed to grow magnetically. This Phase I study will seek to demonstrate the optimal particle characteristics to maximize uptake, using an organotypic technique and in vivo administration to an acutely traumatized rat spinal cord. A further experiment will seek to determine the volume of particles that must be incorporated to enable the axon/particle complex to be directed through a viscous column with a magnetic field gradient of 7000 Gauss.

描述（由申请人提供）：受损的哺乳动物脊髓不会自发自我修复。该项目的目标是开发一种用于轴突生长和脊髓功能恢复的系统，其中聚合物和抗Li磁性纳米颗粒附着在创伤轴突的生长锥上，然后诱导轴突沿着来自外部磁场的磁通线向远端沿着生长。这将代表一种独特的专利方法，涉及（1）一种新的外科技术，（2）一种注射装置，（3）一种产生精确磁场的装置。已经表明，施加于CNS神经元的张力确实刺激定向生长。在以前的研究中，ODI已经表明BSA包被的颗粒被CNS神经元吞噬，并且细胞可以被磁性引导生长。该I期研究将试图证明最佳颗粒特性，以最大限度地吸收，使用器官型技术和体内给药急性创伤大鼠脊髓。进一步的实验将寻求确定必须掺入的颗粒的体积，以使轴突/颗粒复合物能够被引导通过具有7000高斯的磁场梯度的粘性柱。