Developing the Zebrafish as an animal model for aging

开发斑马鱼作为衰老动物模型

基本信息

  • 批准号:
    6684675
  • 负责人:
  • 金额:
    $ 8.11万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2003
  • 资助国家:
    美国
  • 起止时间:
    2003-08-01 至 2004-07-31
  • 项目状态:
    已结题

项目摘要

The focus of the application addresses the topic: Animal Models of Aging, under the National Institute on Aging's priority area of Enhancing Resources to Support High Quality Research. Zebrafish is a relatively new model for aging research, in part because it has been only in the last five to ten years that zebrafish have been used extensively for any type of research. The recent emergence of the zebrafish as a widely studied species now provides an opportunity to exploit this model for aging research. The experimental design is structured to generate gene expression profiles in aging and caloric restricted zebrafish that approximates a currently accepted aging model, the mouse. Caloric restriction (CR) is the only known intervention shown to slow incidence of age-related diseases and extend lifespan in a wide variety of organisms, including rodents and zebrafish. Large-scale microarrays have emerged as a powerful tool to profile gene expression. This approach has been used to study aging and caloric restriction in a number of tissues in the mouse. We will profile gene expression in brain, heart and skeletal muscle from zebrafish subject to short-term caloric restriction, and during aging. Subsequently, we will compare our gene expression profiles to those reported recently in the literature for an animal model having a similar life span, the mouse. While we do not expect the results between animals to be identical, due to the variations in species specific process of aging and the differences in protocols, we do expect to see pathways that are fundamentally applicable to the aging process demonstrate changes that are at least consistent in the direction of change between the two model systems. Comparisons between the two models should provide valuable insights into the aging process and its inhibition by caloric restriction.
该应用程序的重点是解决主题:老化的动物模型,根据国家老龄化研究所的优先领域,加强资源,以支持高质量的研究。斑马鱼是一种相对较新的衰老研究模型,部分原因是斑马鱼只是在过去的五到十年中才被广泛用于任何类型的研究。最近出现的斑马鱼作为一个广泛研究的物种,现在提供了一个机会,利用这种模型的老化研究。实验设计的结构,以产生基因表达谱的老化和热量限制的斑马鱼,接近目前公认的衰老模型,小鼠。热量限制(CR)是唯一已知的干预措施,可以减缓年龄相关疾病的发病率,并延长各种生物体的寿命,包括啮齿动物和斑马鱼。大规模的微阵列已经成为一个强大的工具,以剖析基因表达。这种方法已被用于研究小鼠体内许多组织的衰老和热量限制。我们将分析斑马鱼大脑、心脏和骨骼肌在短期热量限制和衰老过程中的基因表达。随后,我们将我们的基因表达谱与最近在文献中报道的具有相似寿命的动物模型小鼠的基因表达谱进行比较。虽然我们不期望动物之间的结果是相同的,但由于物种特定衰老过程的变化和方案的差异,我们确实期望看到基本上适用于衰老过程的途径表现出至少在方向上一致的变化 两个模型系统之间的变化。这两个模型之间的比较应该提供有价值的见解的老化过程和抑制热量限制。

项目成果

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