SHY2/IAA3 in auxin regulation of plant development

描述(申请人提供)：植物激素生长素(IAA， 吲哚-3-乙酸)是一种关键的信息分子，它控制着许多 植物发育的各个方面，包括特定组织的分化 如木质部、枝条和根的分枝、伸长和向阳 对光和重力的反应。在模式植物拟南芥shy2中 编码该家族成员的IAA3基因发生突变。的研究 这些突变体表明SHY2/IAA3是一个重要的调控因子。 生长素控制发育，也影响对光的反应。这项建议 描述实验以确定SHY2/IAA3如何将光感知和 生长素的反应，以及它如何调节发育。这些方法将包括 Shy2突变表型分析；SHY2/IAA3蛋白相互作用研究 与其他调节蛋白；SHY2/IAA3蛋白修饰的研究， 植物的稳定性和定位；全球基因表达的研究 Shy2和其他突变体的模式；以及遗传修饰物的特征 一种害羞的突变。这些实验将帮助我们了解生长素是如何 在模型系统中规范开发，并将在未来允许 以有益于人类健康的方式操纵农作物的形态。

DESCRIPTION (provided by applicant): The plant hormone auxin (IAA, indole-3-acetic acid) is a crucial informational molecule that controls many aspects of plant development, including differentiation of particular tissues such as xylem, branching of both shoots and roots, and elongation and tropic responses to light and gravity. In the model plant Arabidopsis thaliana, shy2 mutations fall in the IAA3 gene encoding a member of this family. Study of these mutants has shown that SHY2/IAA3 is an important regulator of auxin-controlled development and also affects responses to light. This proposal describes experiments to determine how SHY2/IAA3 may link light perception and auxin responses, and how it regulates development. The approaches will include analyses of shy2 mutant phenotypes; studies of SHY2/IAA3 protein interactions with other regulatory proteins; studies of SHY2/IAA3 protein modification, stability, and localization in plants; studies of global gene expression patterns in shy2 and other mutants; and characterization of genetic modifiers of a shy2 mutation. These experiments will help us understand how auxin regulates development in a model system, and will in the future allow manipulation of morphology of crop plants in ways beneficial to human health.