Study of Lead Exposure & Outcomes Amongst Children in C*

描述(由申请人提供) 这项修订提案中的研究将主要在印度进行，作为国家卫生研究院第#R01 ES07821号补助金的延伸。铅暴露在印度构成了一个重大的环境健康问题。印度还没有进行直接研究来调查铅暴露对神经行为发育等结果的影响。我们建议在拟议的国际和平研究所作为高级富布赖特学者工作期间启动的合作努力的基础上，与印度泰米尔纳德邦金奈(以前称为Madras)的SRI Ramachandra医学院和研究所的同事合作，研究在金奈地区特定公立学校就读的750名4-6岁的儿童。这些学校将被选为代表城市交通和工业高-高、高-低、低-高和低-低的地区。对于每个儿童，我们将测量血液和乳牙中的铅水平；评估视觉空间功能(使用Raven的渐进矩阵)、视觉运动功能(使用广泛的视觉运动能力评估)、行为(使用Conners的评定量表修订)和智商(使用比奈-卡马斯测试)；采集血液进行DNA提取和氨基酮丙酸脱水酶(ALAD)、载脂蛋白E和转铁蛋白基因的基因分型；以及收集有关协变量的数据。 有了这些信息，我们将描述铅暴露和暴露-剂量关系，并测试关于铅剂量与神经行为结果的关系以及基因多态对这些关系的修正作用的具体假设。这项研究承诺提供关于铅毒性的新的科学信息，以及关于铅暴露对印度城市儿童神经行为发育的影响的重要信息。它还将成为美国和印度之间建立合作环境健康研究的一个空白。

DESCRIPTION (provided by applicant) The research in this revised proposal will be performed primarily in India as an extension of NIH grant #R01 ES07821. Lead exposure poses a major environmental health problem in India. No direct studies have yet been performed in India to investigate the impact of lead exposure on outcomes such as neurobehavioral development. We propose to build upon a collaborative effort initiated during the proposed PI's work as a Senior Fulbright Scholar and work with colleagues at the Sri Ramachandra Medical College and Research Institute of Chennai (formerly known as Madras), Tamil Nadu, India, to study 750 children aged 4-6 attending specific public schools in the area of Chennai. These schools will be chose to represent areas of the city that are high-high, high-low, low-high, and low-low with respect to traffic and industry. For each child we will measure levels of lead in blood and shed primary teeth; assess visuospatial function (using Raven's Progressive Matrices), visuomotor function (using the Wide Range Assessment of Visual Motor Abilities), behavior (using Conners' Rating Scale-Revised), and IQ (using the Binet-Kamath test); collect blood for the extraction of DNA and genotyping for polymorphisms of the aminolevulinic acid dehydratase (ALAD), apoliproprotein E, and transferrin genes; and collect data on covariates. With this information we will describe lead exposure and exposure-dose relationships and test specific hypotheses on the relationship of lead dose to neurobehavioral outcomes and the modifying effect of genetic polymorphisms on these same relationships. This research promises to provide novel scientific information on lead toxicity as well as important information on the impact of lead exposure on pediatric neurobehavioral development in urban India. It will also serve as a nidus for building collaborative environmental health research between the U.S. and India.