Femtosecond Laser Enhanced Aqueous Outflow Facility

飞秒激光增强水流出设施

• 批准号: 6784555
• 负责人: TIBOR JUHASZ
• 金额: $ 5.37万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-01 至 2004-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6784555
• 关键词: Macaca mulatta; computer program /software; conjunctiva; disease /disorder model; eye circulation; eye laser surgery; glaucoma; intraocular aqueous flow; laboratory rabbit; lasers; mathematical model; model design /development; morphology; nonhuman therapy evaluation; scanning electron microscopy; sclera; surgery material /equipment; technology /technique development; wound healing

DESCRIPTION (provided by applicant): Femtosecond laser pulses can be delivered through transparent and translucent tissue to perform high precision surgical procedures without damage to superficial or adjacent tissues. These unique properties of femtosecond laser-tissue interactions may provide a marked advantage over traditional laser techniques for high precision subsurface procedures. We hypothesize that the application of femtosecond lasers to the treatment of glaucoma has several potential advantages over traditional surgical and laser treatments. Among these advantages, localized tissue effects, intra-scleral capabilities, highly precise beam delivery through the conjunctiva and sclera, minimally invasive treatments, short procedure times and a potentially decreased healing response are significant. To test our hypothesis we propose the evaluation of femtosecond laser created intra-scleral fistulas of various geometries for the facilitation of aqueous outflow. Initially, a comprehensive in vitro tissue study will be performed to understand the effects femtosecond laser cut fistulas on aqueous outflow and refine geometric requirements. Theoretical modeling of the aqueous outflow dynamics will also be performed in order to deeper understand the flow dynamics and to aid channel optimization. To ensure precise drainage channel creation, ultrasound imaging will be used to monitor the surgical procedures. Ultrasound imaging is a powerful tool that can provide high-resolution visualization of the femtosecond laser treatments in both optically transparent and non-transparent tissue. After the completion of the in vitro investigations animal experiments are planned to study the effect of femtosecond laser created intra-scleral fistulas on the aqueous humor outflow in vivo. Optimization of the experimental apparatus and surgical procedures will be first accomplished, allowing the execution of long-term wound-healing studies. The longevity and effectiveness of the femtosecond laser procedures will be compared to those of traditional surgical and laser therapies tested under similar conditions. The successful completion of this project will provide a proof of concept for a minimally invasive, high precision femtosecond laser treatment of glaucoma. Additionally, by providing information on the in vivo physiological properties of the aqueous humor outflow, the results will add to our understanding of the etiology and mechanisms of glaucoma.

描述（由申请人提供）：飞秒激光脉冲可通过透明和半透明组织输送，以执行高精度外科手术，而不会损伤浅表或邻近组织。飞秒激光-组织相互作用的这些独特性质可以提供优于传统激光技术的显著优势，用于高精度表面下手术。我们假设飞秒激光治疗青光眼与传统手术和激光治疗相比有几个潜在的优势。在这些优点中，局部组织效应、巩膜内能力、通过结膜和巩膜的高精度射束递送、微创治疗、短手术时间和潜在降低的愈合反应是显著的。为了验证我们的假设，我们建议评价飞秒激光创建的各种几何形状的巩膜内瘘，以促进房水流出。 首先，将进行一项全面的体外组织研究，以了解飞秒激光切割瘘管对房水流出的影响，并细化几何要求。还将进行水流出动力学的理论建模，以更深入地了解流动动力学并帮助通道优化。为了确保精确的引流通道创建，将使用超声成像来监测外科手术。超声成像是一种功能强大的工具，可以在光学透明和不透明组织中提供飞秒激光治疗的高分辨率可视化。 体外研究完成后，计划进行动物实验，以研究飞秒激光创建的巩膜内瘘对体内房水流出的影响。首先将完成实验装置和手术程序的优化，以便执行长期的伤口愈合研究。飞秒激光手术的寿命和有效性将与在相似条件下测试的传统手术和激光治疗进行比较。 该项目的成功完成将为青光眼的微创、高精度飞秒激光治疗提供概念验证。此外，通过提供关于房水流出的体内生理特性的信息，结果将增加我们对青光眼的病因和机制的理解。