Multi-labeled material for in vivo cell tracking

用于体内细胞追踪的多标记材料

• 批准号: 6832127
• 负责人: Dennis E Vaccaro
• 金额: $ 9.83万
• 依托单位: BIOPAL, INC
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6832127
• 关键词: activation analysis; biomaterial development /preparation; biomaterial evaluation; biotechnology; cell population study; cell transplantation; colloids; electron microscopy; fluorescence; human therapy evaluation; magnetic resonance imaging; optics; polymers; reagent /indicator; tissue /cell culture

DESCRIPTION (provided by applicant): Cell-based therapies are rapidly gaining acceptance for use in treating a variety of diseases. The development of labeled materials to non-radioactively track therapeutic cells in vivo is needed to measure the efficacy of delivery, bio-distribution and effective dose. This new material(s) is designated "In Vivo Survey Technology" (IVST). IVST is a single tracer material designed to be assayed by a number of measurement technologies and designed to be readily taken up by cell populations in vitro. Assay technologies potentially useful for IVST measurements include neutron activation analysis for quantification, and optical fluorescence, x-ray fluorescence, electron microscopy and magnetic resonance for imaging. Since each technology offers distinct advantages to the researcher in tracking and quantifying cells in vivo, this project's major aim is to develop and test materials that can be detected and/or measured by all these technologies. In Phase I, materials will be tested to optimize for neutron activation analysis and at least two imaging technologies. The Phase II portion of this proposal will focus on tracking labeled cells in animal models to prove that all measurements can be performed with the newly developed IVST material(s) and to develop a variety of coatings that can either target IVST materials to specific cell types or be optimized for uptake into all cells. IVST will be used initially by scientists to determine the quantity and location of cells, such as stem cells, transplanted during pre-clinical research. IVST will allow researchers to conduct analyses on cell therapy not readily feasible previously.

描述(由申请人提供)：基于细胞的疗法正在迅速获得接受，用于治疗各种疾病。为了测量给药效果、生物分布和有效剂量，需要开发无放射性示踪体内治疗细胞的标记材料。这一新材料(S)被指定为“体内调查技术”(IVST)。IVST是一种单一示踪材料，旨在通过多种测量技术进行分析，并易于在体外被细胞群体吸收。可能对IVST测量有用的分析技术包括用于定量的中子激活分析，以及用于成像的光学荧光、X射线荧光、电子显微镜和磁共振。由于每种技术都为研究人员在体内跟踪和量化细胞提供了明显的优势，该项目的主要目标是开发和测试可以通过所有这些技术检测和/或测量的材料。在第一阶段，将对材料进行测试，以优化中子激活分析和至少两种成像技术。该提案的第二阶段将侧重于在动物模型中跟踪标记细胞，以证明所有测量都可以使用新开发的体外受精-胚胎移植材料(S)进行，并开发各种涂层，既可以针对特定类型的体外受精-胚胎移植材料，也可以针对所有细胞的吸收进行优化。IVST最初将被科学家用来确定在临床前研究期间移植的细胞的数量和位置，例如干细胞。IVST将允许研究人员对细胞疗法进行分析，这在以前是不可行的。