Automated Perfused Culture Process for Adult Stem Cells

成体干细胞的自动化灌注培养过程

• 批准号: 6758584
• 负责人: Kristin Goltry
• 金额: $ 23.38万
• 依托单位: AASTROM BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6758584
• 关键词: biomedical automation; biomedical equipment development; cell adhesion; cell differentiation; cell proliferation; clinical research; flow cytometry; growth media; human tissue; perfusion; stem cells; surface coating; technology /technique development; tissue /cell culture; tissue /cell preparation

DESCRIPTION (provided by applicant): Adherent mesenchymal cell populations derived from bone marrow (BM) have the capability of differentiating into multiple cell lineages in vitro and in vivo. The potential use of these populations, referred to as marrow stromal cells (MSC), in clinical applications to treat nonhematopoietic disorders is being intensely investigated. Ex vivo expansion is being used to increase the number of multipotent MSC available for cell and gene therapy applications. There are currently no standardized methodologies for the culture of these cells, however plating and culturing cell densities have been found to be critical in maintaining the multipotentiality of these cells. Aastrom has previously developed a proprietary single-pass perfusion (SPP) process that has successfully expands BM stem cells with demonstrated engraftment capability in humans. This process technology has been effectively implemented in an automated clinical culture system, the AastromReplicell (tm) Cell Production System (ARS), which enables cells to be produced in a single step process without pre-selection or step-cultures. The culture chamber of this system, along with the operating system software, can both be modified to apply for the growth of other cell types. Using this platform technology, the overall goal of the proposed studies is to develop a GMP compliant automated SPP culture process for the generation of large numbers of highly functional MSC suitable for clinical use. This process will be reliable and reproducible, and the unique combination of SPP and closed system automation should allow MSC production under GMP conditions with minimal manipulation. In Phase I, basic culture parameters known to significantly effect adherent cell proliferation and differentiation will be defined in small-scale cultures to optimize the number of functional MSC produced. MSC will be identified by flow cytometry, colony assays, and differentiation assays. Plastic surface characteristics, including roughness, patterning, and tissue-culture treatment, will be defined. Next, several serum-free media formulations and surface coatings will be examined. Initial studies on the effects of medium perfusion and oxygenation levels will also be performed. In Phase II, after further optimization, defined parameters will be used to modify the ARS components, and the expansion process will be validated to confirm feasibility in the automated SPP system

描述(申请人提供)：来自骨髓(BM)的贴壁间充质细胞群体在体外和体内具有分化为多细胞系的能力。这些被称为骨髓基质细胞(MSC)的群体在治疗非造血疾病的临床应用中的潜在用途正在密切研究中。体外扩增正被用于增加可用于细胞和基因治疗应用的多潜能间充质干细胞的数量。目前还没有用于培养这些细胞的标准化方法，然而，培养和培养细胞密度被发现对维持这些细胞的多能性至关重要。阿斯特罗姆此前开发了一种专利的单次灌流(SPP)过程，该过程已成功地扩大了骨髓干细胞，并证明了其在人类体内的植入能力。这项工艺技术已在自动化临床培养系统AstromReplicell(Tm)细胞生产系统(ARS)中得到有效实施，该系统使细胞能够在无需预选或分步培养的情况下在一步工艺中生产。该系统的培养室和操作系统软件都可以进行修改，以适用于其他类型的细胞的生长。利用这一平台技术，本研究的总体目标是开发一种符合GMP的自动SPP培养过程，以产生大量适合临床使用的高功能MSC。这一过程将是可靠的和可重复的，SPP和封闭系统自动化的独特组合应该允许在GMP条件下以最小的操作进行MSC生产。在第一阶段，已知对贴壁细胞增殖和分化有显著影响的基本培养参数将在小规模培养中确定，以优化产生的功能性MSC的数量。骨髓间充质干细胞将通过流式细胞术、集落试验和分化试验进行鉴定。将定义塑料表面特征，包括粗糙度、图案化和组织培养处理。接下来，我们将研究几种无血清介质配方和表面涂层。还将进行有关介质灌流和氧合水平影响的初步研究。在第二阶段，经过进一步的优化，将使用定义的参数对ARS组件进行修改，并验证扩展过程在自动化SPP系统中的可行性