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EXPANSION OF HUMAN UNBILICAL CORD BLOOD STEM CELLS

EXPANSION OF HUMAN UNBILICAL CORD BLOOD STEM CELLS
人脐带血干细胞的扩增
批准号:
2906122
负责人:
Kristin Goltry
金额:
$37.18万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-09-11 至 2003-08-31

项目摘要

项目成果

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中文摘要
翻译
脐带血(CB)作为造血资源的利用
英文摘要
The utilization of umbilical cord blood (CB) as a source of hematopoietic stem cells in transplantation has been limited by low cell numbers in CB samples. Expanding CB cells ex vivo can potentially overcome these limitations. Aastrom has developed a clinical-scale, automated, GMP cell production system (CPS) which can successfully expand CB samples. In Phase II studies, clinical trials are proposed to test the safety and efficacy of using expanded CB to augment standard CB transplants. In parallel, CB expansion conditions will be optimized for maximal output of primitive hematopoietic stem cells. The effects of culture variables such as cytokine combinations, accessory/stromal cells, inoculum density, and medium perfusion rates, on the output of CD34+lin- cells and LTC-IC will be examined. Using optimized conditions, the maintenance of hematopoietic repopulating cells will then be determined using an in vivo NOD/scid mouse model. Based on phase II results, phase III studies will pursue clinical trials using optimally expanded CB in stem cell transplants, employing either modified augmentation protocols or replacement strategies. Successful completion of these trials would lead to the marketing of the Aastrom CPS for CB cell expansion, potentially leading to more widespread use of CB in stem cell transplantation. PROPOSED COMMERCIAL APPLICATIONS: A closed, automated, GMP system for CB cell expansion would be of great value. CB transplants have several advantages over BM or mPB transplants, but are currently limited by the number of cells available. CB cell expansion would alleviate this limitation, thereby facilitating CB transplants for many indications including hematopoietic stem cell transplantation and gene therapy.
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Clinical-scale Production of Osteoprogenitor Cells
  • 批准号:
    6934052
  • 项目类别:
  • 资助金额:
    $32.37万
  • 财政年份:
    2003
  • 负责人:
    Kristin Goltry
  • 依托单位:
Automated Large-Scale Production of Endothelial Cells
  • 批准号:
    6693921
  • 项目类别:
  • 资助金额:
    $9.98万
  • 财政年份:
    2003
  • 负责人:
    Kristin Goltry
  • 依托单位:
Automated Perfused Culture Process for Adult Stem Cells
  • 批准号:
    6758584
  • 项目类别:
  • 资助金额:
    $23.38万
  • 财政年份:
    2003
  • 负责人:
    Kristin Goltry
  • 依托单位:
Automated Perfused Culture Process for Adult Stem Cells
  • 批准号:
    6645557
  • 项目类别:
  • 资助金额:
    $20.88万
  • 财政年份:
    2003
  • 负责人:
    Kristin Goltry
  • 依托单位:
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