Profiling of signal transduction pathways in cancer

癌症信号转导途径的分析

• 批准号: 6734153
• 负责人: SERGEI S MAKAROV
• 金额: $ 14.56万
• 依托单位: ATTAGENE, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-09 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6734153
• 关键词: biological signal transduction; biotechnology; cAMP response element binding protein; cadherins; carcinogenesis; neoplastic process; nuclear factor kappa beta; p53 gene /protein; protein structure function; protooncogene; technology /technique development; transcription factor

DESCRIPTION (provided by applicant): A hallmark of cancer is deregulated activation of the signal transduction pathways controlling proliferation, apoptosis, and differentiation. The signal transduction pathways culminate in activation of transcription factors (TFs), proteins that bind promoter regions of genes, thereby controlling transcription initiation. To understand the complex regulation of the signal transduction network, it is necessary to put in place technologies for assessment of numerous TFs. It is currently possible to monitor the specific changes occurring in cancer cells in the expression and function of genes and gene products at the DNA, RNA, or protein level. However, very few tools are available for examining the molecular changes at the TF level. ATTAGENE has developed a proprietary technology enabling the profiling of the transcriptional activities of multiple TFs in vivo. Based on this technology, we will develop an assay suitable for the profiling of several TFs that are critically involved in carcinogenesis, including c-Myc, E2F, p53, AP-1, NF-kB, CREB, and beta-catenin/TCF. The utility of this assay will be tested in several human cancer cell lines. Our technology allows for a comprehensive analysis of alterations in the signal transduction pathways occurring in cancer and for an identification of TFs that play a key role in a particular type or stage of cancer. The availability of this tool will advance understanding of cancer cell anatomy.

描述(由申请人提供)： 癌症的一个标志是控制增殖、凋亡和分化的信号转导通路的非调控激活。信号转导通路最终激活转录因子，转录因子是一种结合基因启动子区域的蛋白质，从而控制转录启动。为了了解信号转导网络的复杂调节，有必要建立评估大量TF的技术。目前，在DNA、RNA或蛋白质水平上监测癌细胞在基因和基因产物的表达和功能上发生的特定变化是可能的。然而，很少有工具可用于在Tf水平上检查分子变化。Attagene已经开发出一项专利技术，能够在体内分析多个转录因子的转录活动。基于这项技术，我们将开发一种适合于几种在致癌过程中起关键作用的转录因子的分析方法，包括c-Myc、E2F、p53、AP-1、NF-kB、CREB和β-catenin/TCF。这项测试的实用性将在几个人类癌细胞系中进行测试。我们的技术允许对癌症中发生的信号转导途径的变化进行全面分析，并识别在特定类型或阶段的癌症中发挥关键作用的转铁蛋白。该工具的使用将促进对癌细胞解剖学的了解。