RAD51 gene functions in mitosis and meiosis of zebrafish

RAD51 基因在斑马鱼有丝分裂和减数分裂中的作用

• 批准号: 6836127
• 负责人: Hong Ma
• 金额: $ 4.8万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6836127
• 关键词: DNA binding protein; DNA damage; animal genetic material tag; cell cycle; cell growth regulation; chemical stability; chromosomes; embryo /fetus; embryogenesis; gene expression; gene expression profiling; genetic recombination; genetic regulation; genetically modified animals; in situ hybridization; larva; meiosis; protein localization; protein structure function; zebrafish

DESCRIPTION (provided by applicant): Double-stranded breaks (DSBs) in DNA occur in eukaryotes during normal DNA replication, recombination, and DNA damage, but must be repaired to maintain cellular viability. The RAD51 gene was discovered to play a critical role in double strand break repair in the budding yeast S. cerevisiae. Its close homologs and paralogs may also have important functions on DNA repair and chromosome stability in multicellular organisms. The experimental advantages of the zebrafish (Danio rerio) allow us to test two important hypotheses regarding the function of the RAD51 family members in vertebrates: Hypothesis 1: RAD51 and several RAD51 paralogs are expressed in proliferating cells. We will test this hypothesis using whole mount in situ hybridization in various stages of embryo and larval development. Hypothesis 2: Normal levels of expression of at least some RAD51 family members are important for embryonic development and/or genomic instability. We will test this hypothesis using morpholino knockdowns of these genes. These experiments will pave the way for more extensive studies in the near future.

描述（由申请人提供）： DNA双链断裂（DSB）发生在真核生物正常的DNA复制，重组和DNA损伤，但必须修复以维持细胞活力。RAD 51基因在芽殖酵母S.啤酒。它的近缘同源物和旁系同源物也可能在多细胞生物中对DNA修复和染色体稳定性具有重要功能。斑马鱼（Danio rerio）的实验优势使我们能够测试两个重要的假设，关于脊椎动物中的RAD 51家族成员的功能：假设1：RAD 51和几个RAD 51旁系同源物在增殖细胞中表达。我们将在胚胎和幼虫发育的各个阶段使用整体原位杂交来验证这一假设。假设2：至少一些RAD 51家族成员的正常表达水平对于胚胎发育和/或基因组不稳定性是重要的。 我们将使用这些基因的吗啉敲除来检验这一假设。这些实验将为不久的将来进行更广泛的研究铺平道路。