Peptide Models for Type III Copper Sites in Proteins

蛋白质中 III 型铜位点的肽模型

• 批准号: 6837360
• 负责人: DAVID A ROCKCLIFFE
• 金额: $ 4.76万
• 依托单位: TEXAS A&M UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-23 至 2005-08-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6837360
• 关键词: X ray crystallography; active sites; binding sites; conformation; copper; crystallization; electron spin resonance spectroscopy; infrared spectrometry; ligands; macromolecule; model; nuclear magnetic resonance spectroscopy; peptides; solvents; structural biology

DESCRIPTION (provided by applicant): The proposed study involves examining the binding sites of copper (I) and copper (ll) in three peptides with a view to establishing the coordination environment of the metal ion. In the first stage of the project, the solution conformation of the prepared peptides will be examined with and without the presence of copper (I) and copper (ll) ions in order to establish the extent of conformational change that is necessary for complexation of copper. The binding sites of copper in the copper-peptide complexes will be further determined with EPR, ENDOR, ESEEM, NMR and FTIR spectroscopy. Two of the prepared peptides will be reacted with an appropriate copper salt in a 2:1 copper (I)/peptide ratio in order to produce a dicopper complex. The dicopper complex will be transformed into a dioxygen adduct through reaction with molecular oxygen. The dioxygen complex for each ligand will be investigated by the use of electronic absorption spectroscopy and NMR spectroscopy. Stability of the dioxygen complex towards water and other protic solvents will be examined. Physical studies, including X-ray structural analysis, are proposed for all isolated complexes of the oxy or deoxy forms.

描述（由申请人提供）：拟议的研究涉及检查三种肽中铜（I）和铜（II）的结合位点，以建立金属离子的配位环境。在该项目的第一阶段，将在铜 (I) 和铜 (II) 离子存在和不存在的情况下检查所制备的肽的溶液构象，以确定铜络合所需的构象变化程度。铜-肽复合物中铜的结合位点将通过 EPR、ENDOR、ESEEM、NMR 和 FTIR 光谱进一步确定。两种制备的肽将与适当的铜盐以 2:1 的铜 (I)/肽比例反应，以产生二铜络合物。二铜配合物将通过与分子氧反应转化为双氧加合物。每个配体的双氧配合物将通过使用电子吸收光谱和核磁共振光谱进行研究。将检查双氧络合物对水和其他质子溶剂的稳定性。建议对所有孤立的氧或脱氧形式的复合物进行物理研究，包括 X 射线结构分析。