Role of myosin-1 in membrane and actin dynamics

myosin-1 在膜和肌动蛋白动力学中的作用

• 批准号: 6835864
• 负责人: CHRISTINA A KING-SMITH
• 金额: $ 4.45万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-16 至 2006-06-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6835864
• 关键词: actins; cell line; cell membrane; cell motility; chemical kinetics; immunocytochemistry; membrane activity; molecular biology; myosins; neoplastic cell culture for noncancer research; protein isoforms; protein localization; protein protein interaction; protein structure function; small interfering RNA

DESCRIPTION (provided by applicant): The objectives of the proposed research are to understand the roles of myosin I in membrane retraction and retrograde actin flow. Myosin Is represent one of the largest classes of unconventional myosins in humans, yet little is known about their activity, distribution, or regulation in vertebrate cells. Specific aims include the following: 1) To characterize the distribution of myo1c during lamellipodial extension and retraction in MTLn3 cells, a metastatic mammalian cell line. This will be accomplished by immunofluorescence microscopy and transient expression of eGFP-myosin fusion proteins. 2) To determine the effect of myosin-I "knockdown" on cell spreading, membrane retraction, and retrograde actin flow using small interfering RNA (siRNA). Retrograde flow in control and myosin-I knockdown cells will be assessed by motility of surface-attached latex beads. 3) To determine the relationship between the mechanical activity of myo1c and membrane dynamics. We will express myo1c mutants that have altered motor properties, and we will examine the effect of these mutants on membrane dynamics.

描述（由申请人提供）：