MECHANISMS OF ACTIN DEPENDENT ORGANELLE MOTILITY

肌动蛋白依赖的细胞器运动机制

• 批准号: 2842282
• 负责人: CHRISTINA A KING-SMITH
• 金额: $ 9.23万
• 依托单位: SAINT JOSEPH'S UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2842282
• 关键词: Osteichthyes; actins; apical membrane; cellular polarity; electron microscopy; granule; intracellular transport; microfilaments; myosins; organelles; pigments; protein localization; stainings

DESCRIPTION (adapted from investigator's abstract): The objective of the proposed research is to characterize the mechanism of actin-dependent pigment granule motility in RPE cells of teleosts. A model proposing that centripetal aggregation of pigment granules is linked to centripetal flow of actin filaments while centrifugal dispersion of pigment granules is affected along actin filaments via myosin motors, will be tested. Specific aims are to 1) characterize the ultrastuctural organization of F actin in RPE cells, 2) determine the polarity of actin filaments in RPE apical projections via S1 labeling, 3) determine whether particles attached to the surface of RPE cells undergo centripetal transport and 4) localize myosins VI and VIIa in RPE cells.

说明(改编自调查员摘要)： 建议的研究是为了表征肌动蛋白依赖的色素的机制。 硬骨鱼RPE细胞中的颗粒运动。一个提出向心力的模型 色素颗粒的聚集与肌动蛋白的向心流有关 长丝对颜料颗粒的离心分散有影响 通过肌球蛋白马达的肌动蛋白细丝将被测试。具体目标为1) RPE细胞中F-肌动蛋白的超微结构组织特征，2) 通过S1确定RPE根尖投射中肌动蛋白细丝的极性 标记，3)确定颗粒是否附着在RPE细胞表面 进行向心转运和4)在RPE细胞中定位肌球蛋白VI和VIIa。