Vision Research Infrastructure Development

描述（由申请人提供）： 虽然路易斯维尔大学眼科和视觉科学系拥有大量NEI资助的视觉科学家，但它以前没有核心模块服务的资金。 该应用程序的目的是为我们的科学家提供额外的共享支持，以提高他们的个人研究能力，并加强进一步的合作。此外，它旨在通过鼓励合作研究和吸引其他科学家进行视觉研究来提高路易斯维尔大学进行视觉研究的研究能力。这些目标将通过运作两个研究单元来实现，这两个单元将提供以下服务： L.图像采集和分析模块将提供以下方面的技术支持和专业知识： 组织学程序，例如常规组织学处理、处理冷冻组织 样本/块和免疫组织化学染色，共聚焦显微镜等。此外，它将 在数字图像的获取、处理和分析方面提供协助和专业知识 使用各种成像设备如共焦、明场和荧光显微镜捕获。 二.流式细胞术模块将帮助研究者识别混合细胞群中的特定细胞类型。此类研究包括但不限于T细胞亚群的估计、有丝分裂状态的测定以及细胞表面受体和标志物的研究。 这些服务和资源将通过扩展NEI资助科学家的能力，促进合作和吸引新科学家参与视觉研究来加强路易斯维尔大学的视觉研究。

DESCRIPTION (provided by applicant): Although the Department of Ophthalmology and Visual Sciences at the University of Louisville has a large cadre of NEI funded vision scientists, it has not previously had funding for core module services. The purpose of this application is to provide our scientists with additional shared support to enhance their individual research capabilities and enhance further collaboration. Additionally, it is oriented to enhance the research capability of the University of Louisville for conducting vision research by encouraging collaborative studies and attracting other scientists to vision research. These goals will be met by operating two research modules which will provide the following services: l. The Image Acquisition and Analysis Module will provide technical support and expertise in histological procedures such as conventional histological processing, processing frozen tissue samples/blocks and immunohistochemical staining, confocal microscopy, etc. Additionally it will provide assistance and expertise in the acquisition, manipulation and analysis of digital images captured using various imaging devices such as confocal, bright field and fluorescence microscopy. II. The Flow Cytometry Module will help investigators identify specific cell types in mixed cell populations. Such studies include, but are not limited to, estimation of subsets of T-cells, determination of mitotic state and studies of cell surface receptors and markers. These services and resources will enhance vision research at the University of Louisville by extending the capability of NEI funded scientists, promoting collaboration and attracting new scientist to vision research.