RESPONSE OF THE AGING NERVOUS SYSTEM TO TRAUMA

老化神经系统对创伤的反应

• 批准号: 6922014
• 负责人: STEPHEN W SCHEFF
• 金额: $ 35.65万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6922014
• 关键词: adenosine triphosphate; aging; brain injury; cognition; creatine; diet therapy; dietary supplements; disease /disorder model; free fatty acids; laboratory rat; lactates; mitochondria; neurochemistry; nonhuman therapy evaluation; peroxidation; psychological aspect of aging

DESCRIPTION (provided by applicant): Abundant clinical data indicate increased morbidity and mortality following trauma to the aging nervous system. Little is currently known about the cellular substrates underlying this amplified adverse response. The proposed studies lead to testing of the hypothesis, that age-related changes associated with traumatic brain injury (TBI) can be negated by enhancing the ability of neurons to produce ATP. This idea is based on the theory that age-related changes in brain mitochondria lead to a disruption of ATP necessary to meet the energy demands of neurons following trauma. Mitochondria are the major source of ATP required for neuronal function. Age-related defects in mitochondrial oxidative phosphorylation result in deceased energy production, impaired cellular calcium buffering, activation of proteases and phospholipases, and the generation of increased free radicals. All of these pathways can lead to enhanced cell death depending on the severity of the insult. Preliminary data from our laboratory demonstrate age-related differences following experimental TBI, supporting its usefulness as an aging animal model. We also present data that a diet supplemented by creatine provides a neuroprotective intervention for TBI. Because of a lack of sufficient information on aged animal models of TBI, this study will first characterize age-related changes. Specific aim #1 will characterize age-related decline in morphologic and behavioral changes following TBI employing an animal model of controlled cortical contusion. Specific aim #2 will characterize age-related changes in synaptic and non-synaptic mitochondria following TBI. Specific aim #3 will explore age-related changes in the generation of lactic acid and free fatty acids, sensitive markers of secondary injury, and changes in isoprostanes and neuroprostanes, markers of lipid peroxidation following TBI. Specific aim #4 will use a 'creatine-supplemented' dietary intervention that enhances cytosolic phosphocreatine and increases the ability of neurons to produce ATP, an intervention to reverse the age-related response to TBI.

描述（由申请人提供）：大量临床数据表明，老化神经系统创伤后发病率和死亡率增加。目前对这种放大的不良反应的细胞底物知之甚少。拟议的研究导致测试的假设，即与创伤性脑损伤（TBI）相关的年龄相关的变化可以通过增强神经元产生ATP的能力来否定。这个想法是基于这样的理论，即大脑线粒体中与年龄相关的变化导致ATP的破坏，而ATP是满足创伤后神经元能量需求所必需的。线粒体是神经元功能所需的ATP的主要来源。线粒体氧化磷酸化中的线粒体相关缺陷导致能量产生减少、细胞钙缓冲受损、蛋白酶和磷脂酶活化以及自由基产生增加。所有这些途径都可以导致增强的细胞死亡，这取决于损伤的严重程度。我们实验室的初步数据表明，实验性TBI后存在年龄相关的差异，支持其作为衰老动物模型的有用性。我们还提供了数据表明，补充肌酸的饮食可以为TBI提供神经保护干预。由于缺乏足够的信息，老年动物模型的TBI，本研究将首先表征年龄相关的变化。具体目标#1将采用受控皮质挫伤的动物模型表征TBI后形态和行为变化的年龄相关性下降。具体目标#2将表征TBI后突触和非突触线粒体中的年龄相关变化。具体目标#3将探索乳酸和游离脂肪酸生成的年龄相关变化，继发性损伤的敏感标志物，以及TBI后脂质过氧化的标志物异前列腺素和神经前列腺素的变化。具体目标#4将使用“补充肌酸”的饮食干预，其增强细胞溶质磷酸肌酸并增加神经元产生ATP的能力，这是一种逆转对TBI的年龄相关反应的干预。