Plant terpenoids: Deciphering metabolic pathways and improving production in microbes
植物萜类化合物:破译代谢途径并提高微生物的产量
基本信息
- 批准号:10714595
- 负责人:
- 金额:$ 39.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-01 至 2028-06-30
- 项目状态:未结题
- 来源:
- 关键词:AccelerationBiochemicalBiological AssayBiomanufacturingDigitalis (genus)EngineeringGene ClusterGenomicsKnowledgeLifeMedicineMetabolic PathwayMethodsMicrobeMilletMiningPathway interactionsPlant ExtractsPlant GenomePlantsPrevalenceProductionProductivityPublic HealthResearchRouteSaffronSourceTerpenesTimeWorkYeastsanalogcost effectivehuman diseaseimprovedmetabolomicsnovelpreventprogramsreconstitutiontranscriptomics
项目摘要
Beyond their prevalence in everyday life, many plant terpenoids have life-saving medicinal applications for a
variety of human diseases. Despite their clear medicinal importance, it remains unclear how plants synthesize
most plant terpneoids. This knowledge gap prevents us from developing a better method to access terpenoids
since medicinal terpenoids are extracted from plants with meager yields. This research program aims to
accelerate the identification of plant terpenoid biosynthetic pathways and to improve plant terpenoid production
in microbes. Synthesizing plant terpenoids in microbes is a far more cost-effective approach as it can significantly
enhance terpenoid productivity and shorten production time. We developed an integrated workflow capable of
high-throughput characterization of multiple plant terpenoid pathways in parallel. This workflow combines
genomic mining, transcriptomic analysis, metabolomics, and biochemical assays for terpenoid pathway
elucidation followed by pathway reconstitution in engineered microbes for high-titer terpenoid production. Our
workflow is unique in that it includes gene clusters in plant genomes as an untapped source for uncovering novel
plant biosynthetic pathways. Another unique feature of our workflow is that we use a highly engineered yeast
strain as a platform to produce plant specialized terpenoids. This highly productive platform strain sets the stage
for cost-effective production of any of the >80,000 terpenoids and infinite numbers of terpenoid analogs. In this
research program, we will apply this workflow to decipher multiple plant terpenoid pathways, including valuable
mono-, di-, sesqui-, tri-, and tetra-terpenoid synthetic routes in foxglove, millet, crocus, and other plant species.
Progress from the proposed work will advance fundamental knowledge regarding how plants synthesize
medicinally important or biologically active terpenoids. It will also transform the biomanufacturing of plant-based
medicines for renewable and cost-effective production.
除了在日常生活中普遍存在外,许多植物萜类化合物还具有挽救生命的药用价值
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Zhen Wang其他文献
Zhen Wang的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Zhen Wang', 18)}}的其他基金
Synergistic interactions of hypertension and diabetes in promoting kidney injury
高血压和糖尿病协同作用促进肾损伤
- 批准号:
9295148 - 财政年份:2017
- 资助金额:
$ 39.88万 - 项目类别:
相似海外基金
CAREER: Biochemical and Structural Mechanisms Controlling tRNA-Modifying Metalloenzymes
职业:控制 tRNA 修饰金属酶的生化和结构机制
- 批准号:
2339759 - 财政年份:2024
- 资助金额:
$ 39.88万 - 项目类别:
Continuing Grant
Leveraging releasable aryl diazonium ions to probe biochemical systems
利用可释放的芳基重氮离子探测生化系统
- 批准号:
2320160 - 财政年份:2023
- 资助金额:
$ 39.88万 - 项目类别:
Standard Grant
Diurnal environmental adaptation via circadian transcriptional control based on a biochemical oscillator
基于生化振荡器的昼夜节律转录控制的昼夜环境适应
- 批准号:
23H02481 - 财政年份:2023
- 资助金额:
$ 39.88万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Systematic manipulation of tau protein aggregation: bridging biochemical and pathological properties
tau 蛋白聚集的系统操作:桥接生化和病理特性
- 批准号:
479334 - 财政年份:2023
- 资助金额:
$ 39.88万 - 项目类别:
Operating Grants
Converting cytoskeletal forces into biochemical signals
将细胞骨架力转化为生化信号
- 批准号:
10655891 - 财政年份:2023
- 资助金额:
$ 39.88万 - 项目类别:
Enhanced Biochemical Monitoring for Aortic Aneurysm Disease
加强主动脉瘤疾病的生化监测
- 批准号:
10716621 - 财政年份:2023
- 资助金额:
$ 39.88万 - 项目类别:
Biochemical Mechanisms for Sustained Humoral Immunity
持续体液免疫的生化机制
- 批准号:
10637251 - 财政年份:2023
- 资助金额:
$ 39.88万 - 项目类别:
Structural and biochemical investigations into the mechanism and evolution of soluble guanylate cyclase regulation
可溶性鸟苷酸环化酶调节机制和进化的结构和生化研究
- 批准号:
10604822 - 财政年份:2023
- 资助金额:
$ 39.88万 - 项目类别:
Chemical strategies to investigate biochemical crosstalk in human chromatin
研究人类染色质生化串扰的化学策略
- 批准号:
10621634 - 财政年份:2023
- 资助金额:
$ 39.88万 - 项目类别:
Examination of risk assessment and biochemical assessment of fracture development focusing on the body composition of patients with rheumatoid arthritis
关注类风湿性关节炎患者身体成分的骨折发生风险评估和生化评估检查
- 批准号:
22KJ2600 - 财政年份:2023
- 资助金额:
$ 39.88万 - 项目类别:
Grant-in-Aid for JSPS Fellows














{{item.name}}会员




