Understanding and Targeting the R-Loop-Mediated DNA Damage Response at Telomeres

了解并靶向 R 环介导的端粒 DNA 损伤反应

• 批准号: 10716512
• 负责人: Li Lan
• 金额: $ 36.83万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-10 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10716512
• 关键词: Aging; Bypass; Cell Aging; Cell division; Cells; Chemicals; Cockayne Syndrome; Critical Pathways; Cytoprotection; DNA Damage; DNA Repair Pathway; DNA biosynthesis; DNA lesion; DNA replication fork; Dependence; Future; Genomic Instability; Human; Investigation; Lesion; Link; Longevity; Malignant Neoplasms; Mediating; Molecular; Mus; Pathway interactions; Patients; Phenotype; Premature aging syndrome; Process; Proteins; RAD52 gene; Radiation; Reactive Oxygen Species; Role; Source; Stress; Telomerase; Telomere Pathway; Telomere Shortening; Testing; Untranslated RNA; anticancer research; cancer cell; cancer type; cell age; clinical investigation; coping; novel; oxidative DNA damage; oxidative damage; pre-clinical; protective pathway; repaired; response; senescence; sensor; targeted cancer therapy; telomere; telomere loss; tumorigenesis

Project Summary Oxidative DNA damage is frequently generated by radiation, chemicals, and endogenous oxygen radicals, contributing to genomic instability during both aging and tumorigenesis. Oxidative damage at telomeres can lead to telomere loss or attrition, which triggers cellular senescence and limits the lifespan of dividing cells. While it is clear that oxygen radicals can inflict multiple types of DNA lesions, how these lesions are repaired at telomeres is still largely unknown. By inducibly and locally generating reactive oxygen species (ROS) at telomeres, we discovered a novel DNA repair pathway critical for the protection of cells against telomeric oxidative damage. This pathway is activated by ROS-induced R-loops, and is mediated by break-induced replication (BIR), a process that “jumpstarts” DNA synthesis at collapsed replication forks. In parallel with our studies on the oxidative damage response at telomeres, we also investigated how cancer cells maintain telomeres to bypass senescence. In particular, we have molecularly dissected the alternative lengthening of telomere (ALT) pathway, which is used by ~10-15% of human cancers to extend telomeres. Interestingly, we found that ALT is also an R-loop-triggered and BIR-mediated repair pathway. The unexpected similarities between the repair pathway dealing with telomeric oxidative damage and the ALT pathway lead us to hypothesize that these telomere repair pathways are mechanistically linked. Furthermore, cancer cells hijack the R-loop and BIR-mediated repair pathway to extend telomeres and bypass senescence. In Aim 1, we will systematically delineate the R-loop and BIR-mediated pathway that repairs telomeric oxidative damage, and investigate if this pathway contributes to ALT activation in cancer cells. In Aim 2, we will develop strategies to exploit the cellular dependency on the R-loop and BIR-mediated ALT pathway, which may allow us to selectively kill ALT+ cancer cells and aged cells harboring high telomeric oxidative damage. Our studies may establish a new link between cellular aging and tumorigenesis, and provide new opportunities to eliminate cancer cells by targeting a hijacked DNA repair pathway. These studies may have transformative impacts at the interface between aging and cancer research, opening a new avenue to future preclinical and clinical investigations.

项目摘要 DNA氧化损伤通常是由辐射、化学物质和内源性氧自由基引起的， 在衰老和肿瘤形成过程中都会导致基因组的不稳定。端粒的氧化损伤可能 导致端粒丢失或磨损，从而触发细胞衰老，限制细胞分裂的寿命。 虽然氧自由基可以造成多种类型的DNA损伤是显而易见的，但这些损伤是如何修复的 端粒在很大程度上仍不为人所知。通过诱导和局部产生活性氧物种(ROS) 端粒，我们发现了一种新的DNA修复途径，对保护细胞免受端粒的攻击至关重要 氧化损伤。该通路由ROS诱导的R-环激活，并由Break诱导 复制(BIR)，这是一个在折叠的复制叉处“启动”DNA合成的过程。与我们的 关于端粒氧化损伤反应的研究，我们还研究了癌细胞如何维持 端粒可以绕过衰老。特别是，我们已经从分子上剖析了另一种延长方式 端粒(ALT)途径，约10%-15%的人类癌症用来延长端粒。有趣的是，我们 发现ALT也是R环触发的BIR介导的修复途径。出乎意料的相似之处 在处理端粒氧化损伤的修复途径和ALT途径之间引导我们 假设这些端粒修复途径是机械连接的。此外，癌细胞劫持了 R环和BIR介导的修复途径延长端粒和绕过衰老。在目标1中，我们将 系统地描绘了R环和BIR介导的修复端粒氧化损伤的途径，以及 研究这一途径是否有助于癌细胞中ALT的激活。在目标2中，我们将制定战略，以 利用细胞对R环和BIR介导的ALT途径的依赖性，这可能允许我们 选择性地杀死ALT+癌细胞和具有高端粒氧化损伤的老化细胞。我们的研究可能 在细胞衰老和肿瘤发生之间建立新的联系，并提供新的机会消除 通过锁定被劫持的DNA修复途径来抑制癌细胞。这些研究可能会产生变革性的影响 衰老和癌症研究之间的接口，为未来的临床前和临床开辟了一条新的途径 调查。