ACT 2 PROJ 1: EFFECTS OF DIETARY SELENIUM ON THE DEVELOPMENT OF ASTHMA

第 2 步项目 1：膳食硒对哮喘发生的影响

• 批准号: 7561549
• 负责人: PETER R HOFFMANN
• 金额: $ 15.47万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561549
• 关键词: Acute; Affect; African American; Antibodies; Antioxidants; Asthma; CCL4 gene; Chronic; Clinical Trials; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Dietary Selenium; Dose; Exhibits; Funding; Goals; Grant; Histology; Human; Institution; Intake; Irrigation; Measures; Messenger RNA; Minority Groups; Mus; Ovalbumin; Play; Prevention; Proteins; Research; Research Personnel; Resources; Role; Selenium; Serum; Source; Structure of parenchyma of lung; United States; United States National Institutes of Health; airway remodeling; cytokine; design; ethnic minority population; feeding; health disparity; human study; mouse model; research study; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Asthma affects an estimated 31 million people in the United States. African Americans and other ethnic minority groups are disproportionately over-represented among those suffering with asthma. Recent data have suggested that dietary selenium (Se), a potent antioxidant, may play an important role in the prevention of asthma. The long-term goal of this research is to reduce the human suffering and health disparities from asthma. The objective of the project is to employ a mouse model to determine how levels of dietary selenium affect the development of acute and chronic asthma. Determining the relationship between dietary Se and asthma would provide a strong scientific framework for eventual clinical trials in humans. The development of asthma in the mouse model is evaluated by: histology of lung tissue sections; bronchiolar lavage to establish cellular and cytokine profiles; mRNA and protein levels of various factors involved in asthma and airway remodeling; and levels of anti-ovalbumin antibodies in bronchiolar lavage and serum. Using these measures, we have demonstrated that Se intake and asthma are not related in a simple dose-response manner in an experiment designed to examine acute asthma. That is, we have shown that mice in the medium-Se group exhibit the most severe asthma, while those fed low or high Se develop less severe asthma. These results may help to explain inconsistent findings in human studies that failed to establish the relationship between dietary Se and asthma.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 据估计，美国有 3100 万人患有哮喘。 非裔美国人和其他少数族裔在哮喘患者中所占比例过高。 最近的数据表明，膳食中的硒（Se）是一种有效的抗氧化剂，可能在预防哮喘方面发挥重要作用。这项研究的长期目标是减少哮喘给人类带来的痛苦和健康差异。该项目的目标是利用小鼠模型来确定膳食硒水平如何影响急性和慢性哮喘的发展。 确定膳食硒与哮喘之间的关系将为最终的人体临床试验提供强有力的科学框架。 通过以下方式评估小鼠模型中哮喘的发展：​​肺组织切片的组织学；细支气管灌洗以建立细胞和细胞因子谱；哮喘和气道重塑涉及的各种因子的 mRNA 和蛋白质水平；以及细支气管灌洗液和血清中抗卵清蛋白抗体的水平。通过这些措施，我们在一项旨在检查急性哮喘的实验中证明了硒摄入量与哮喘之间不存在简单的剂量反应关系。也就是说，我们已经证明，中硒组的小鼠表现出最严重的哮喘，而喂食低硒或高硒的小鼠则表现出较不严重的哮喘。这些结果可能有助于解释人类研究中未能确定膳食硒与哮喘之间关系的不一致结果。