Cellular Immunology of Enterovirus Infection

肠道病毒感染的细胞免疫学

• 批准号: 7220846
• 负责人: PAUL A KROGSTAD
• 金额: $ 1.39万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2007-06-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7220846
• 关键词: Acute Myocarditis; Anatomy; Animals; Award; CD4 Positive T Lymphocytes; CD8B1 gene; Cellular Immunity; Cellular Immunology; Childhood; Coxsackie B Viruses; Coxsackie Viruses; Disease; Encephalitis; Engineering; Enteral; Enterovirus; Enterovirus Infections; Epitopes; Family; Family Picornaviridae; Fellowship; Foot-and-Mouth Disease Virus; Foundations; Goals; Hepatitis A Virus; Human; Human poliovirus; Immune; Immune response; Immunology; Infection; Intestines; Investigation; Laboratories; Lymphocytic choriomeningitis virus; Modeling; Mucosal Immune Responses; Mus; Myocarditis; Oral; Outcome; Pancreatitis; Pathogenesis; Picornaviridae Infections; Polioviruses; Reagent; Recombinants; Role; Route; Specificity; T-Cell Receptor; T-Lymphocyte; T-Lymphocyte Epitopes; Transgenic Mice; Variant; Viral; base; intraperitoneal; member; pathogen; research study; response; tissue tropism; vector vaccine

DESCRIPTION (provided by applicant): Members of the picornavirus family are responsible for a wide variety of diseases among humans and other animal species. While the replication of picornaviruses is generally well understood, little is known about cellular immune mechanisms elicited by picornavirus infection. Coxsackie B virus (CVB) is a member of the enterovirus genus of picornaviruses. The general goal of the studies proposed is to characterize the breadth and magnitude of cellular immune responses elicited by CVB infection, using murine models of enteral and parenteral infection. The specific aims are to quantify and characterize CD4 and CD8 T cell responses elicited following oral inoculation, and to compare these to cellular immune responses elicited by parenteral infection. These experiments will employ recombinant CVB variants engineered to express lymphocytic choriomeningitis virus (LCMV) epitopes, and transgenic mice that express a specific LCMV specific TCR variant. These studies will provide a foundation for further studies of host-pathogen relationships in picornavirus disease and the potential utility of picornaviruses as enterally delivered vaccine vectors.

描述（由申请人提供）：小核糖核酸病毒家族的成员是人类和其他动物物种中各种疾病的罪魁祸首。虽然人们对小核糖核酸病毒的复制已经有了很好的了解，但对小核糖核酸病毒感染引发的细胞免疫机制知之甚少。柯萨奇B型病毒（CVB）是小核糖核酸病毒肠病毒属的一员。提出的研究的总体目标是表征CVB感染引起的细胞免疫反应的广度和程度，使用小鼠肠内和肠外感染模型。具体目的是量化和表征口服接种后引起的CD4和CD8 T细胞反应，并将其与肠外感染引起的细胞免疫反应进行比较。这些实验将使用重组CVB变体来表达淋巴细胞性脉络丛脑膜炎病毒（LCMV）表位，以及表达LCMV特异性TCR变体的转基因小鼠。这些研究将为进一步研究小核糖核酸病毒病的宿主-病原体关系以及小核糖核酸病毒作为肠内递送疫苗载体的潜在效用提供基础。