DEVELOPMENTAL EXPRESSION OF COXSACKIE ADENOVIRUS RECEPTOR

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Coxsackievirus B and Adenoviruses are the most common viral pathogens implicated in the development of human myocarditis, and gain entry into cells via the Coxsackie Adenovirus Receptor (CAR). We examined CAR expression in non-human primates, using RT- PCR, immunoblot, and immunofluorescence. These data suggest indicate that myocarditis and other manifestations of CVB infection in newborns may reflect higher CAR expression. Baseline ECG, antibody levels to coxsackievirus, and echocardiograms were obtained in 8 cynomolgus monkeys. Initial training for minimally invasive cardiac biopsy techniques has been completed. Initially, two monkeys were inoculated IV with 108 pfu of cocsackie virus. Both animals showed clinical signs of disease and had histologic evidence of virus-induced lesions in multiple organs. Because the initial infection caused such a severe, acute infection the next two animals received a reduced dose of virus. On was inoculated IV and the second received an oral/gastric exposure. Both animals became infected but the orally inoculated animal appeared to clear the infection within 2 weeks. The third pair of animals received an IV inoculation of 107 pfu of coxsackie virus and were followed for virema and development of cardiac lesions for 4 weeks post-inoculation. Inoculation of the final two monkeys will occur in the next few weeks pending virology and pathology results from the last two animals. Current results indicate that cynomolgus macaques are a useful model of coxsackie virus infection in humans.