Enzymology of RNA Processing Enzymes

RNA加工酶的酶学

基本信息

  • 批准号:
    7161780
  • 负责人:
  • 金额:
    $ 25.56万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1997
  • 资助国家:
    美国
  • 起止时间:
    1997-01-01 至 2009-12-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Ribonuclease P (RNase P) catalyzes the maturation of the 5' end of precursor tRNA (pre-tRNA) to form tRNA, a component essential for the synthesis of proteins. RNase P from bacteria is composed of an RNA subunit that catalyzes pre-tRNA cleavage in vitro and a protein component that is essential for activity in vivo and enhances binding of the pre-tRNA substrate. In contrast, RNase P in eukaryotes contains one RNA and multiple protein subunits. We propose to investigate the function of the bacterial RNase P using a combination of biochemical and structural techniques. Specifically, we aim to: (1) explore the structure and dynamics of RNase P using time resolved fluorescence resonance energy transfer techniques to measure distances and mobility; (2) investigate substrate recognition in bacterial RNas P by determining the thermodynamics and function of PRNA-pre-tRNA and pre-tRNA-P protein contacts in RNase P and investigating the cleavage of novel RNA substrates; (3) investigate the position and functions of metals bound to RNase P for both catalysis and substrate recognition; and (4) delineate the position of metal ion binding sites in RNase P and the structure of isolated helices by NMR spectroscopic analysis. Our long term goal is to further understand (1) the mechanisms of catalysis used by ribozymes as compared to protein enzymes, and (2) the structure and energetics of RNA binding proteins and protein/RNA complexes. RNase P is a unique enzyme to investigate catalytic strategies and substrate recognition since the active site is near the protein-RNA interface. This unique collaboration between the protein and RNA subunits may provide insight into the evolution from RNA to protein catalysts. RNase P is an essential enzyme as tRNA maturation is required for protein synthesis. RNase P has potential medical applications as a novel antibiotic target since it is an essential enzyme and the eukaryotic and prokaryotic enzymes have different subunit composition. The structural and functional studies proposed here should provide insight into the development of active site-directed inhibitors of bacterial RNase P from target organisms such as S. aureus and Bacillus anthracis.
描述(申请人提供):核糖核酸酶P(RNaseP)催化前体tRNA(前-tRNA)的5‘端成熟形成tRNA,这是蛋白质合成所必需的成分。细菌RNaseP由一个在体外催化Pre-tRNA裂解的RNA亚基和一个对体内活性至关重要并增强Pre-tRNA底物结合的蛋白质成分组成。相反,真核生物中的核糖核酸酶P含有一个RNA和多个蛋白质亚基。我们建议使用生化和结构技术相结合的方法来研究细菌RNaseP的功能。具体来说,我们的目标是:(1)利用时间分辨荧光共振能量转移技术测量距离和迁移率,探索RNaseP的结构和动力学;(2)通过测定RNaseP中PRNA-pre-tRNA和Pre-tRNA-P蛋白质接触的热力学和功能以及研究新的RNA底物的切割,研究细菌RNAs P中的底物识别;(3)研究与RNaseP结合的金属在催化和底物识别方面的位置和功能;以及(4)通过核磁共振光谱分析确定RNaseP中金属离子结合位点的位置和分离螺旋的结构。我们的长期目标是进一步了解(1)核酶相对于蛋白质酶的催化机制,以及(2)与RNA结合的蛋白质和蛋白质/RNA复合体的结构和能量。RNaseP是一种独特的研究催化策略和底物识别的酶,因为它的活性部位靠近蛋白质-RNA界面。蛋白质和RNA亚基之间的这种独特的合作可能为我们提供从RNA到蛋白质催化剂的进化的洞察力。RNaseP是一种必需的酶,因为蛋白质合成需要tRNA的成熟。核糖核酸酶P是一种重要的酶,且真核和原核酶具有不同的亚基组成,因此作为一种新型的抗生素靶标具有潜在的医疗应用价值。这里提出的结构和功能研究应该为从金黄色葡萄球菌和炭疽芽孢杆菌等靶生物中开发活性位点定向的细菌核糖核酸酶P抑制剂提供洞察力。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

CAROL A FIERKE其他文献

CAROL A FIERKE的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('CAROL A FIERKE', 18)}}的其他基金

Disruption of transition metal homeostasis by Cd: Implications for aging
镉对过渡金属稳态的破坏:对衰老的影响
  • 批准号:
    8056614
  • 财政年份:
    2010
  • 资助金额:
    $ 25.56万
  • 项目类别:
Disruption of transition metal homeostasis by Cd: Implications for aging
镉对过渡金属稳态的破坏:对衰老的影响
  • 批准号:
    7874096
  • 财政年份:
    2010
  • 资助金额:
    $ 25.56万
  • 项目类别:
ENZYMOLOGY OF RNA PROCESSING ENZYMES
RNA 加工酶的酶学
  • 批准号:
    6138553
  • 财政年份:
    1997
  • 资助金额:
    $ 25.56万
  • 项目类别:
ENZYMOLOGY OF RNA PROCESSING ENZYMES
RNA 加工酶的酶学
  • 批准号:
    6044649
  • 财政年份:
    1997
  • 资助金额:
    $ 25.56万
  • 项目类别:
Enzymology of RNA Processing
RNA 加工的酶学
  • 批准号:
    8402158
  • 财政年份:
    1997
  • 资助金额:
    $ 25.56万
  • 项目类别:
ENZYMOLOGY OF RNA PROCESSING ENZYMES
RNA 加工酶的酶学
  • 批准号:
    2023953
  • 财政年份:
    1997
  • 资助金额:
    $ 25.56万
  • 项目类别:
ENZYMOLOGY OF RNA PROCESSING ENZYMES
RNA 加工酶的酶学
  • 批准号:
    6152279
  • 财政年份:
    1997
  • 资助金额:
    $ 25.56万
  • 项目类别:
ENZYMOLOGY OF RNA PROCESSING ENZYMES
RNA 加工酶的酶学
  • 批准号:
    6627210
  • 财政年份:
    1997
  • 资助金额:
    $ 25.56万
  • 项目类别:
ENZYMOLOGY OF RNA PROCESSING ENZYMES
RNA 加工酶的酶学
  • 批准号:
    6693084
  • 财政年份:
    1997
  • 资助金额:
    $ 25.56万
  • 项目类别:
Enzymology of RNA Processing
RNA 加工的酶学
  • 批准号:
    8600688
  • 财政年份:
    1997
  • 资助金额:
    $ 25.56万
  • 项目类别:

相似海外基金

Construction of affinity sensors using high-speed oscillation of nanomaterials
利用纳米材料高速振荡构建亲和传感器
  • 批准号:
    23H01982
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Affinity evaluation for development of polymer nanocomposites with high thermal conductivity and interfacial molecular design
高导热率聚合物纳米复合材料开发和界面分子设计的亲和力评估
  • 批准号:
    23KJ0116
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Development of High-Affinity and Selective Ligands as a Pharmacological Tool for the Dopamine D4 Receptor (D4R) Subtype Variants
开发高亲和力和选择性配体作为多巴胺 D4 受体 (D4R) 亚型变体的药理学工具
  • 批准号:
    10682794
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
Platform for the High Throughput Generation and Validation of Affinity Reagents
用于高通量生成和亲和试剂验证的平台
  • 批准号:
    10598276
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
Collaborative Research: DESIGN: Co-creation of affinity groups to facilitate diverse & inclusive ornithological societies
合作研究:设计:共同创建亲和团体以促进多元化
  • 批准号:
    2233343
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
    Standard Grant
Collaborative Research: DESIGN: Co-creation of affinity groups to facilitate diverse & inclusive ornithological societies
合作研究:设计:共同创建亲和团体以促进多元化
  • 批准号:
    2233342
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
    Standard Grant
Molecular mechanisms underlying high-affinity and isotype switched antibody responses
高亲和力和同种型转换抗体反应的分子机制
  • 批准号:
    479363
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
    Operating Grants
Deconstructed T cell antigen recognition: Separation of affinity from bond lifetime
解构 T 细胞抗原识别:亲和力与键寿命的分离
  • 批准号:
    10681989
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
CAREER: Engineered Affinity-Based Biomaterials for Harnessing the Stem Cell Secretome
职业:基于亲和力的工程生物材料用于利用干细胞分泌组
  • 批准号:
    2237240
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
    Continuing Grant
ADVANCE Partnership: Leveraging Intersectionality and Engineering Affinity groups in Industrial Engineering and Operations Research (LINEAGE)
ADVANCE 合作伙伴关系:利用工业工程和运筹学 (LINEAGE) 领域的交叉性和工程亲和力团体
  • 批准号:
    2305592
  • 财政年份:
    2023
  • 资助金额:
    $ 25.56万
  • 项目类别:
    Continuing Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了