Chromatically Orthogonal Photolabile Protecting Groups for the Parallel On-Chip Synthesis of High-Density Glycan Microarrays
用于并行片上合成高密度聚糖微阵列的色谱正交光不稳定保护基团
基本信息
- 批准号:10722250
- 负责人:
- 金额:$ 14.86万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-08-01 至 2025-07-31
- 项目状态:未结题
- 来源:
- 关键词:AccelerationAntibodiesBODIPYBacteriaBenignBindingBinding ProteinsBiological ProcessCarbohydratesCollaborationsColorCombinatorial SynthesisComplexDNADNA SequenceDarknessDevelopmentDyesEpitope MappingEpitopesFamilyFluorescenceGene ChipsGenotypeHealthHumanImmuneIn SituIndividualLabelLectinLibrariesLightMatched GroupMethodsMonitorMonosaccharidesNeoplasm MetastasisOligonucleotidesOutcomes ResearchPhotochemistryPolymersPolysaccharidesReagentResearchS phaseSignal TransductionSourceSpecificityStructureStructure-Activity RelationshipSurfaceTechnologyViralVirusWorkabsorptionchromophorecombinatorialdensityexperienceexperimental studyfabricationgene synthesisglycosylationirradiationmanufacturemicrochippathogenscreeningsugartrafficking
项目摘要
Abstract. The objective of this research is to identify carbohydrate-compatible photolabile protecting groups
and light sources to facilitate the parallel on-surface synthesis of high-density glycan microarrays—e.g. glycan
chips—in a manner similar to the synthesis of gene chips. Instead of using monochromatic light and a single
photolabile protecting group to spatially control the extension of a linear polymer (DNA) on the microchip, as in
gene chip manufacture, irradiation with different wavelengths of light combined with wavelength-selective tem-
porary protecting groups will allow for constructing complex, branched glycans on a microchip surface. I aim to:
(1) identify carbohydrate-compatible photolabile protecting groups and optimal light sources to pair with each;
and (2) identify a pair of wavelength-selective (`chromatically orthogonal') protecting groups and demonstrate
their use in branched glycan synthesis, paving the way towards the parallel on-chip synthesis of high-density
glycan microarrays. Wavelength-selective photochemistry will be achieved by separating the absorptions of pho-
tolabile protecting groups to allow for selective excitation. Such high-density combinatorially-synthesized glycan
chips are expected to permit rapid epitope mapping and screening of the selectivity of glycan binding partners.
For example, exposing a dye-labeled lectin, antibody, or virus/bacteria/pathogen binding protein to the chip will
allow its binding specificity for numerous glycan structures to be determined from a single experiment. The ability
to synthesize high-density combinatorial libraries of carbohydrates will aid in resolving the structure-function
relationships of carbohydrates, help to understand the target epitopes of glycan binding partners, and accelerate
efforts to uncover the structure and function of the glycome.
摘要。本研究的目的是鉴定碳水化合物相容的光性保护基团
项目成果
期刊论文数量(0)
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Arthur H. Winter其他文献
Aryl Nitrenium and Oxenium Ions with Unusual High-Spin π,π* Ground States: Exploiting (Anti)Aromaticity.
具有异常高自旋 π,π* 基态的芳基氮和氧离子:利用(反)芳香性。
- DOI:
- 发表时间:
2017 - 期刊:
- 影响因子:3.6
- 作者:
Yunfan Qiu;Logan J Fischer;Andrew S Dutton;Arthur H. Winter - 通讯作者:
Arthur H. Winter
A radical spin on viologen polymers: organic spin crossover materials in water.
紫罗碱聚合物的激进旋转:水中的有机旋转交叉材料。
- DOI:
10.1039/c4cc07119k - 发表时间:
2015 - 期刊:
- 影响因子:4.9
- 作者:
M. Juetten;Alexander T Buck;Arthur H. Winter - 通讯作者:
Arthur H. Winter
Aromatics from pyrones: esters of terephthalic acid and isophthalic acid from methyl coumalate
来自吡喃酮的芳烃:由香豆酸甲酯生成的对苯二甲酸和间苯二甲酸的酯
- DOI:
10.1039/c3ra42287a - 发表时间:
2013 - 期刊:
- 影响因子:3.9
- 作者:
G. Kraus;G. R. Pollock;Christie L. Beck;Kyle Palmer;Arthur H. Winter - 通讯作者:
Arthur H. Winter
A structurally compact aqueous soluble oxypicolinium photocage with high photosensitivity
一种具有高光敏性的结构紧凑的水溶性氧化吡啶鎓光笼
- DOI:
10.1039/d5sc00204d - 发表时间:
2025-03-11 - 期刊:
- 影响因子:7.400
- 作者:
Komadhie C. Dissanayake;Mohammad K. I. Walid;Madelyn Austin;Emily A. Smith;Arthur H. Winter - 通讯作者:
Arthur H. Winter
Tryptophan and ATTO 590: mutual fluorescence quenching and exciplex formation.
色氨酸和 ATTO 590:相互荧光猝灭和激基复合物形成。
- DOI:
10.1021/jp412045m - 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
U. Bhattacharjee;Christie L. Beck;Arthur H. Winter;C. Wells;J. Petrich - 通讯作者:
J. Petrich
Arthur H. Winter的其他文献
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