Campylobacter jejuni restriction by the intestinal microbiota

项目说明 空肠弯曲杆菌是美国食源性腹泻最常见的原因。虽然它是最多的 通常与自限性腹泻疾病有关，一小部分感染患者会发展成更多 严重的神经退行性并发症称为格林-巴利综合征。尽管有许多重要的 尽管该领域取得了很大进展，但人们对其致病机制仍知之甚少。空肠弯曲菌 能够有效地在大范围宿主的肠道中定居，从而使该病原体能够进入 对食物链的影响，从而给食品工业带来了重大挑战。人们普遍观察到， 在有充分记录的点源暴发或受控人类感染期间，空肠弯曲菌的发病率低于 50%，而且即使在严重暴露的条件下，也只有一小部分人出现症状 感染。众所周知，在生理条件下，栖息的微生物群对 对肠道致病菌的影响。因此，我们假设文件中记录的一些差异 对空肠弯曲菌感染的敏感性是由于肠道微生物区系的组成不同所致。我们 打算进行实验以更好地了解驻留的肠道微生物群的机制 限制空肠弯曲菌的定植。为了实现这一目标，我们计划利用无菌鸡肠 我们最近在我们的实验室中建立了殖民模型，以及我们在 空肠弯曲菌的研究。这些研究将为理解居民的作用提供基础 空肠弯曲菌肠道定植中的微生物区系可作为新药开发的基础 抗击空肠弯曲菌感染的策略。

PROJECT DESCRIPTION Campylobacter jejuni is the most common cause of food-born diarrhea in the United States. Although it is most often associated with self-limiting diarrheal disease, a small proportion of infected patients develop a more serious neuro-degenerative complication known as Guillain-Barré Syndrome. Despite many important advances in the field, still remarkably little is known about its mechanisms of pathogenicity. C. jejuni has the ability to efficiently colonize the intestine of a broad range of hosts, which allows this pathogen to gain access to the food chain thus presenting major challenges to the food industry. It has been widely observed that the attack rate of C. jejuni during well-documented point-source outbreaks or controlled human infections is under 50%, and that even under conditions of heavy exposure, only a subset of individuals developed symptomatic infections. It is well established that under physiological conditions, the resident microbiota exerts a restricting effect over bacterial enteropathogens. We therefore hypothesize that some of the documented differences in susceptibility to C. jejuni infection is due to differences in the composition of the intestinal microbiota. We intend to conduct experiments to better understand the mechanisms by which the resident intestinal microbiota restricts C. jejuni colonization. To achieve this objective we plan to leverage a germ-free chicken gut colonization model that we have recently established in our laboratory along with our long-standing expertise in the study of C. jejuni. These studies will provide the foundation for the understanding of the role of the resident microbiota in C. jejuni intestinal colonization, which may serve as the bases for the development of novel strategies to combat C. jejuni infections.