Campylobacter jejuni restriction by the intestinal microbiota

项目描述 空肠弯曲菌是美国最常见的食源性腹泻原因。虽然它是最 通常与自限性结肠炎有关，一小部分感染患者会出现更多的 严重的神经退行性并发症，称为格林-巴利综合征。尽管有许多重要的 尽管该领域取得了很大进展，但对其致病机制仍知之甚少。C.空肠有 能够有效地定植在各种宿主的肠道中，这使得这种病原体能够进入 因此对食品工业提出了重大挑战。人们普遍认为， C.在有充分记录的点源爆发或控制的人类感染期间， 50%，即使在严重暴露的条件下，也只有一部分人出现症状， 感染.已经确定的是，在生理条件下，常驻微生物群发挥限制作用， 对细菌性肠道病原体的作用。因此，我们假设，一些记录的差异， 对C.空肠感染是由于肠道微生物群组成的差异。我们 打算进行实验，以更好地了解肠道微生物群落的机制， 限制C.空肠定植。为了实现这一目标，我们计划利用无菌鸡肠 殖民化模型，我们最近在我们的实验室沿着与我们长期的专业知识， C.空肠。这些研究将为理解居民的作用提供基础 C.空肠肠道定植，这可能是开发新的 战略打击C。空肠感染

PROJECT DESCRIPTION Campylobacter jejuni is the most common cause of food-born diarrhea in the United States. Although it is most often associated with self-limiting diarrheal disease, a small proportion of infected patients develop a more serious neuro-degenerative complication known as Guillain-Barré Syndrome. Despite many important advances in the field, still remarkably little is known about its mechanisms of pathogenicity. C. jejuni has the ability to efficiently colonize the intestine of a broad range of hosts, which allows this pathogen to gain access to the food chain thus presenting major challenges to the food industry. It has been widely observed that the attack rate of C. jejuni during well-documented point-source outbreaks or controlled human infections is under 50%, and that even under conditions of heavy exposure, only a subset of individuals developed symptomatic infections. It is well established that under physiological conditions, the resident microbiota exerts a restricting effect over bacterial enteropathogens. We therefore hypothesize that some of the documented differences in susceptibility to C. jejuni infection is due to differences in the composition of the intestinal microbiota. We intend to conduct experiments to better understand the mechanisms by which the resident intestinal microbiota restricts C. jejuni colonization. To achieve this objective we plan to leverage a germ-free chicken gut colonization model that we have recently established in our laboratory along with our long-standing expertise in the study of C. jejuni. These studies will provide the foundation for the understanding of the role of the resident microbiota in C. jejuni intestinal colonization, which may serve as the bases for the development of novel strategies to combat C. jejuni infections.