Modernization of Housing for the Peromyscus Genetic Stock Center, an International Research Resource

国际研究资源白鼠遗传资源中心住房的现代化

• 批准号: 10734169
• 负责人: Shayne C Barlow
• 金额: $ 34.62万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10734169
• 关键词: Acclimatization; Adverse effects; Allergens; Animals; Breeding; COVID-19 pandemic; Collection; Data; Deer Mouse; Developmental Biology; Discipline; Epigenetic Process; Equipment; Exhibits; Exposure to; Funding; Genetic; Health; Health Status; Housing; Human; Human Resources; Hypersensitivity; Incidence; Individual; Infection; International; Laboratory Rat; Laboratory mice; Methods; Mission; Modernization; Mus; Peromyscus; Pilot Projects; Policies; Polypropylenes; Procedures; Recording of previous events; Research; Research Personnel; Resources; Risk; Rodent; Scientist; Side; South Carolina; System; Time; Training; United States National Institutes of Health; Universities; Work; biosecurity; disease transmission; experience; germ free condition; high efficiency particulate air filter; improved; laboratory facility; operation; pathogen; pressure; ventilation

Modernization of Housing for the Peromyscus Genetic Stock Center For over 60 years, the University of South Carolina (UofSC) has maintained the Peromyscus Genetic Stock Center (PGSC), a unique international research resource utilized by many NIH-funded scientists from a variety of disciplines including genetics, epigenetics, developmental biology, and transmission of infectious disease. The mission of the PGSC is to maintain colonies of various species of outbred, genetically diverse deer mice (genus Peromyscus) and distribute them to investigators around the world. Today, we actively maintain six different closed specific pathogen free (SPF) colonies. The PGSC is the only living stock collection in the world that maintains and supplies Peromyscus mice. The PGSC originally housed Peromyscus in open-top, opaque polypropylene housing. In 2010, microisolator tops were incorporated along the use of HEPA-filtered changing stations and biosafety cabinets to protect personnel from allergens and improve the health status of the colony. Endemic infections were eliminated to generate SPF Peromyscus mice for research. In 2019, the PGSC began conducting pilot studies to evaluate the effects of IVC housing on various Peromyscus species. After housing Peromyscus mice for over two years on IVC housing, we have determined that: 1. Peromyscus exhibit no adverse effects from being housed on IVC racks 2. Peromyscus maintained or improved breeding efficiency on IVC racks 3. IVC housing resolved health conditions that were believed to be normal for the species. The PGSC wishes to convert the entire colony to IVC housing. We are requesting funds to purchase additional IVC housing sufficient to convert the remainder of the PGSC colony from static microisolator housing. Modernization of the housing conditions in this international research resource will: 1. Protect personnel from allergens and potential pathogens 2. Increase biosecurity and protect the SPF status of the animals 3. Allow expansion of colony size due to increased housing capacity while maintaining the same footprint 4. Significantly increase operational efficiency of the PGSC

Peromyscus遗传资源中心的住房现代化 60多年来，南卡罗来纳州大学（UofSC）一直维持着Peromyscus遗传储备中心（PGSC）， 一个独特的国际研究资源，由许多NIH资助的科学家使用，来自不同学科，包括 遗传学、表观遗传学、发育生物学和传染病传播。PGSC的使命是 维持各种远系繁殖的遗传多样性鹿鼠（鹿鼠属）的种群，并将它们分布到 世界各地的调查员。今天，我们积极维护六个不同的封闭无特定病原体（SPF）菌落。的 PGSC是世界上唯一一个饲养和供应Peromyscus小鼠的活种群。 PGSC最初将Peromyscus安置在顶部开放的不透明聚丙烯外壳中。2010年，微隔离器顶部 沿着使用高效微粒空气过滤器更换站和生物安全柜，以保护工作人员免受过敏原的侵害 改善殖民地的健康状况。消除地方性感染以产生SPF Peromyscus小鼠， research. 2019年，PGSC开始进行试点研究，以评估IVC圈养对各种Peromyscus物种的影响。 在IVC饲养条件下饲养Peromyscus小鼠超过2年后，我们确定： 1.饲养在IVC架上对鹿鼠无不良影响 2.在IVC架上保持或提高了鹿鼠的繁殖效率 3. IVC圈养解决了被认为是该种属正常的健康状况。 PGSC希望将整个殖民地改为IVC住房。我们正在申请资金购买更多的IVC住房 足以将PGSC菌落的剩余部分从静态微隔离器外壳转化。住房现代化 这一国际研究资源的条件将： 1.保护人员免受过敏原和潜在病原体的侵害 2.提高生物安全性并保护动物的SPF状态 3.在保持相同的占地面积的同时，由于增加了住房容量， 4.显著提高PGSC的运营效率