Human Decidual Lymphocytes and their Placental Ligands

人蜕膜淋巴细胞及其胎盘配体

• 批准号: 7149145
• 负责人: JACK L STROMINGER
• 金额: $ 34.99万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-15 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7149145
• 关键词: Allogenic; Authorization documentation; Biochemistry; Cells; Cities; Decidua; Disclosure; Face; Graft Rejection; Granulocyte-Macrophage Colony-Stimulating Factor; Granulocyte-Macrophage Colony-Stimulating Factor Receptors; Histocompatibility Antigens Class I; Human; Human Resources; Immune response; Instruction; Last Name; Ligands; Lymphocyte; Maternal-Fetal Exchange; Mothers; Names; Natural Killer Cells; Numbers; Patients; Placenta; Placentation; Postdoctoral Fellow; Pre-Eclampsia; Pregnancy; Pregnancy Maintenance; Pregnancy loss; Principal Investigator; Printing; Protein Overexpression; Proteins; Recurrence; Research; Research Project Grants; Role; Site; Testing; Universities; cell type; cytokine; cytotrophoblast; fetal; implantation; novel; programs; receptor

The specific aims of this research are to examine the receptors, ligands, and functions of human decidual lymphocytes with a view to understanding their possible roles in implantation and pregnancy. In particular, we wish." . To examine the biochemistry and possible functions of novel proteins overexpressed by human decidual natural killer (NK) cells that may be involved in down-modulation of the immune response, to continue analysis of the transcriptional profiles of these cells and to study their cytoxicity and ability to secrete cytokines. . To assess the role of invariant NKT (iNKT) cells in the decidua by studying their interaction with CDld expressed on several cell types, by examining the relation of their V_ repertoire to cytokine secretion, by testing the hypothesis that they function to regulate the immune response at the maternal-fetal interface and by examining their complete transcriptional profiles. . To examine the expression in the placenta of putative Class I MHC protein ligands for decidual NK and iNKT cells and of the GM-CSF receptor and to examine the interaction of placental extravillous cytotrophoblasts with NK and iNKT cells and with GM-CSF.

本研究的具体目的是检测人蜕膜组织中的受体、配体和功能， 淋巴细胞，以了解它们在着床和妊娠中的可能作用。我们尤其 倒好了" .检测人蜕膜组织中过表达的新蛋白的生物化学和可能的功能， 可能参与免疫应答下调的自然杀伤（NK）细胞，以继续分析 这些细胞的转录谱，并研究其细胞毒性和分泌细胞因子的能力。 .通过研究不变NKT（iNKT）细胞与CDld的相互作用，评估其在蜕膜中的作用。 通过检测它们的V_库与细胞因子分泌的关系， 假设它们的功能是调节母胎界面的免疫反应， 检查它们的完整转录谱 .检测蜕膜NK和iNKT的推定I类MHC蛋白配体在胎盘中的表达 细胞和GM-CSF受体，并检查胎盘绒毛外细胞滋养层细胞与 NK和iNKT细胞以及GM-CSF。