Point of care detection of HPV in saliva
唾液中 HPV 的护理点检测
基本信息
- 批准号:10761543
- 负责人:
- 金额:$ 32.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-12 至 2024-09-11
- 项目状态:已结题
- 来源:
- 关键词:Base SequenceBiochemicalBiological AssayBiological MarkersBiosensorCancerousCaringChemical EngineeringClinicalClustered Regularly Interspaced Short Palindromic RepeatsDNADNA amplificationDataDentistsDetectionDevelopmentDevicesDiagnosisDiagnosticDiseaseEarly DiagnosisElectrodesEngineeringEnsureEnzymesFutureGenesGenetic MaterialsGoalsGoldGuide RNAHPV-High RiskHousekeepingHousekeeping GeneHuman PapillomavirusHuman papilloma virus infectionHuman papillomavirus 16Human papillomavirus 18IncidenceLaboratoriesMalignant NeoplasmsMassachusettsMedicalMethodsMicrofluidic MicrochipsMicrofluidicsMonitorMonitoring for RecurrenceMorbidity - disease rateMultiple PartnersNucleic AcidsOropharyngeal Squamous Cell CarcinomaPathologistPatientsPerformancePersonal SatisfactionPhasePlant LeavesPoint of Care TechnologyPredictive ValuePreparationProgram DevelopmentROC CurveReagentRecurrenceResourcesReverse Transcriptase Polymerase Chain ReactionSalivaSamplingSensitivity and SpecificitySerumSextingSignal TransductionSmall Business Innovation Research GrantSore ThroatSpecimenSymptomsSystemTechnologyTest ResultTestingTimeUnited StatesUnited States National Institutes of HealthValidationViralVirus DiseasesWorkamplification detectionassay developmentcommercializationcostdesigndetection assaydetection sensitivitydetection testdiagnostic assaydiagnostic platformeffective therapyendonucleasehigh riskhigh risk populationimprovedinnovationinventionmalignant oropharynx neoplasmmanufacturemanufacturing process developmentmortalitymultiplex detectionnon-invasive monitornovelnucleic acid deliverynucleic acid detectionparticleplasmid DNApoint of carepoint of care testingpoint-of-care detectionprecision medicineprocess repeatabilityproduct developmentprogramspromote resilienceprototyperelapse patientsscreeningsuccesstechnology platformtranslational medicinetreatment responseviral detectionvirus genetics
项目摘要
Expanded point-of-care (POC) screening and monitoring options for detection of disease biomarkers from easily
collectable specimens such as saliva are needed to reduce the incidence and mortality rates of debilitating
diseases and enhance the resilience of the medical system, and improve care. InnoTech Precision Medicine
with Massachusetts Institute of Technology is working to develop an innovative diagnostic platform that can be
used for POC screening and monitoring of disease biomarkers. In our initial proof-of-concept, we are developing
a POC test for detection of high-risk HPV in saliva. HPV is a viral infection that causes most Oropharyngeal
squamous cell carcinoma (OPSCC) cases. Recent studies have demonstrated that HPV can be detected in
saliva from patients with OPSCC. Currently, the initial screening and diagnosis of OPSCC is done at the late
symptomatic stage based on symptoms that are often mild and can mimic less serious conditions such as cold
or sore throat, delaying diagnosis and treatment. DNA tests for HPV are most often based on RT-PCR detection
of the viral genetic material from serum or saliva that is collected and sent to central laboratories. RT-PCR-based
tests rely on the amplification and detection of viral genetic material. These tests have long actionable turn-
around-time, and high cost. Thus, there are no rapid, accurate and low-cost tests for routine monitoring of high-
risk groups (i.e., young, sextually active, multiple partner) and monitoring of response to therapy and recurrence.
Thus, a POC test for non-invasive monitoring of HPV in saliva during well-being exams can have a significant
impact and save lives. Our objective is to develop a rapid, easy-to-use prototype for the detection of HPV in
saliva based on the combination of biochemical signal amplification with CRISPR-Cas 12 electrochemical
readout and our proprietary robust, accurate and low-cost gold-leaf electrodes. Utilizing our proprietary gold-leaf
electrode along with a CRISPR-Cas12 assay for sensitive and specific electrochemical detection of target nucleic
acid (NA) biomarkers, we have already demonstrated the detection of HPV 18 with plasmid DNA as well as in
patient specimen. Our overall objective for this proposal is to develop a reproducible process for processing of
saliva and develop a prototype electrochemical biosensor for the multiplex detection of HPV 16 and HPV 18
along with a reference gene to ensure accurate test results. To accomplish this goal, we will first develop a
workflow for spatial multiplexing of our gold leaf electrodes for detection of HPV16, HPV 18 and an optimal
reference gene. We will then verify this multiplexed test initially with plasmid DNA, and then with saliva
specimens. We anticipate that combining easy-to-fabricate spatially multiplexed electrodes with CRISPR-
Cas12a for the detection of a specific nucleic acid sequence will enable our Phase II development, regulatory
approval and commercialization of a point-of-need technology that can be scaled and manufactured for accurate,
easy-to-use and low-cost point-of-need testing and to reduce the burden of HPV infection and OPSCC.
扩展的护理点(POC)筛选和监测选项,可轻松检测疾病生物标志物
项目成果
期刊论文数量(0)
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{{ truncateString('ROYA KHOSRAVI-FAR', 18)}}的其他基金
A multimodal platform for Oral screening of COVID-19
用于 COVID-19 口腔筛查的多模式平台
- 批准号:
10665346 - 财政年份:2022
- 资助金额:
$ 32.97万 - 项目类别:
A multimodal platform for Oral screening of COVID-19
用于 COVID-19 口腔筛查的多模式平台
- 批准号:
10266378 - 财政年份:2020
- 资助金额:
$ 32.97万 - 项目类别:
Novel Strategies for Treatment of Myeloproliferative Disorders
治疗骨髓增殖性疾病的新策略
- 批准号:
7670523 - 财政年份:2008
- 资助金额:
$ 32.97万 - 项目类别:
Novel Strategies for Treatment of Myeloproliferative Disorders
治疗骨髓增生性疾病的新策略
- 批准号:
8271290 - 财政年份:2008
- 资助金额:
$ 32.97万 - 项目类别:
Novel Strategies for Treatment of Myeloproliferative Disorders
治疗骨髓增生性疾病的新策略
- 批准号:
8092728 - 财政年份:2008
- 资助金额:
$ 32.97万 - 项目类别:
Novel Strategies for Treatment of Myeloproliferative Disorders
治疗骨髓增生性疾病的新策略
- 批准号:
7523599 - 财政年份:2008
- 资助金额:
$ 32.97万 - 项目类别:
Novel Strategies for Treatment of Myeloproliferative Disorders
治疗骨髓增生性疾病的新策略
- 批准号:
7846141 - 财政年份:2008
- 资助金额:
$ 32.97万 - 项目类别:
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