DISPOSABLE BIOSENSORS FOR NOVEL BIOMARKERS OF RESPONSE

用于响应新型生物标志物的一次性生物传感器

• 批准号: 7851503
• 负责人: Yuehe Lin
• 金额: $ 27.05万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7851503
• 关键词: 3-nitrotyrosine; Antibodies; Apoferritin; Biological; Biological Assay; Biological Markers; Biosensor; Blood; Chemicals; Chronic; Clinical; Complex; Cotinine; Data; Detection; Devices; Disease; Dose; Environmental Exposure; Enzyme-Linked Immunosorbent Assay; Exposure to; Fibrinogen; Goals; Gonadotropins; Health; Human; Immune; Immunoassay; Inflammation; Label; Laboratories; Link; Measures; Methods; Mus; Myoglobin; Nature; Nicotine; Nitrates; Organ; Oxidative Stress; Performance; Plasma; Post-Translational Protein Processing; Proteins; Reference Values; Rest; Saliva; Sampling; Screening procedure; Signal Transduction; Site; Solutions; Stress; System; Techniques; Test Result; Testing; Tobacco smoke; Troponin I; Tumor Necrosis Factor-alpha; Urine; base; biological adaptation to stress; candidate marker; cytokine; environmental agent; environmental stressor; human TNF protein; improved; inorganic phosphate; instrument; nanoparticle; novel; programs; prototype; rapid detection; response; seal; sensor; stressor

The project will develop a portable microanalytical system based on nanoparticle labels/ immunochromatographic electrochemical biosensor (IEB) for on-site rapid detection and quantification of tobacco smoke exposure (TSE)-induced multiple biomarkers in biological matrices. The detection of TSEinduced biomarkers is based on the principle of immunochromatographic separation and nanoparticleenhanced bioelectrochemical detection. To improve the sensitivity of electrochemical detection in the immunochromatographic platform, apoferritin-templated metallic phosphate nanoparticle labels will be employed to amplify detection signals of those biomarkers. The first goal of this Project is to establish nanoparticle-based competitive electrochemical immunoassay for (a) cotinine as a marker of tobacco smoke exposure. The detection system will be tested and evaluated using human plasma and urine samples (Project 1) and mouse plasma, urine, and saliva (Project 2). Secondly, Project 3 will establish nanoparticlebased multiplexed electrochemical immunoassay for sequence specific nitrotyrosine, sequence - independent nitrotyrosine (identified by Project 1 and 2, and Core A and B) and the pleiotropic, non-specific marker, TNF-alpha. Finally, this Project will build a prototype Immunochromatographic/ Electrochemical Biosensor (IEB) device for on-site application. The device will provide for analysis of exposure (cotinine), selected markers of specific (nitrated proteins) and general markers of stress (e.g. TNF-alpha) in a single platform.

该项目将开发一种基于纳米颗粒标记的便携式微量分析系统。 免疫层析电化学生物传感器（IEB）用于现场快速检测和定量 烟草烟雾暴露（TSE）诱导的生物基质中的多种生物标志物。TSE诱导的检测 生物标志物是基于免疫层析分离和纳米颗粒增强的原理 生物电化学检测为了提高电化学检测的灵敏度， 免疫层析平台，脱铁铁蛋白模板的金属磷酸盐纳米颗粒标记将被 用于放大那些生物标志物的检测信号。该项目的第一个目标是建立 基于纳米颗粒的竞争性电化学免疫测定法用于（a）作为烟草烟雾标记物的可替宁 exposure.检测系统将使用人血浆和尿液样本进行测试和评估 （项目1）和小鼠血浆、尿液和唾液（项目2）。第二，项目3将建立基于纳米颗粒的 用于序列特异性硝基酪氨酸、序列特异性硝基酪氨酸和序列特异性硝基酪氨酸的多重电化学免疫测定 独立的硝基酪氨酸（由项目1和2，以及核心A和B鉴定）和多效性，非特异性 标记物，TNF-α。最后，本项目将建立一个原型免疫色谱/电化学 用于现场应用的生物传感器（IEB）装置。该设备将提供暴露分析（可替宁）， 选择的特异性标志物（硝化蛋白）和一般应激标志物（例如TNF-α）在单个 平台