DISPOSABLE BIOSENSORS FOR NOVEL BIOMARKERS OF RESPONSE

该项目将开发一种基于纳米颗粒标记/的便携式微量分析系统 免疫层析电化学生物传感器用于现场快速检测和定量 烟草烟雾暴露(TSE)诱导生物基质中的多个生物标志物。体感诱发电位的检测 生物标志物是基于免疫层析分离和纳米颗粒增强的原理 生物电化学检测。为了提高电化学检测的灵敏度 免疫层析平台，载铁蛋白-模板金属磷酸盐纳米颗粒标记 用于放大这些生物标志物的检测信号。该项目的第一个目标是建立 基于纳米颗粒的可替宁作为烟草烟雾标志物的竞争电化学免疫分析 曝光。检测系统将使用人体血浆和尿液样本进行测试和评估 (项目1)和小鼠血浆、尿液和唾液(项目2)。其次，项目3将建立基于纳米颗粒的 序列特异性硝基酪氨酸的多重电化学免疫分析 独立的硝基酪氨酸(由项目1和2以及核心A和B确定)和多效性、非专一性 标记物，肿瘤坏死因子-α。最后，该项目将建立一个免疫层析/电化学原型 用于现场应用的生物传感器(IEB)装置。该设备将提供暴露(可替宁)的分析， 特定的应激标志物(硝化蛋白)和一般的应激标志物(例如，肿瘤坏死因子-α) 站台。

The project will develop a portable microanalytical system based on nanoparticle labels/ immunochromatographic electrochemical biosensor (IEB) for on-site rapid detection and quantification of tobacco smoke exposure (TSE)-induced multiple biomarkers in biological matrices. The detection of TSEinduced biomarkers is based on the principle of immunochromatographic separation and nanoparticleenhanced bioelectrochemical detection. To improve the sensitivity of electrochemical detection in the immunochromatographic platform, apoferritin-templated metallic phosphate nanoparticle labels will be employed to amplify detection signals of those biomarkers. The first goal of this Project is to establish nanoparticle-based competitive electrochemical immunoassay for (a) cotinine as a marker of tobacco smoke exposure. The detection system will be tested and evaluated using human plasma and urine samples (Project 1) and mouse plasma, urine, and saliva (Project 2). Secondly, Project 3 will establish nanoparticlebased multiplexed electrochemical immunoassay for sequence specific nitrotyrosine, sequence - independent nitrotyrosine (identified by Project 1 and 2, and Core A and B) and the pleiotropic, non-specific marker, TNF-alpha. Finally, this Project will build a prototype Immunochromatographic/ Electrochemical Biosensor (IEB) device for on-site application. The device will provide for analysis of exposure (cotinine), selected markers of specific (nitrated proteins) and general markers of stress (e.g. TNF-alpha) in a single platform.